Relationship of neutrophil phagocytosis and oxidative burst with the subgingival microbiota of generalized aggressive periodontitis

被引:26
作者
Carvalho, R. P. M. [1 ]
Mesquita, J. S. [2 ]
Bonomo, A. [2 ,3 ]
Elsas, P. X. [2 ]
Colombo, A. P. V. [4 ]
机构
[1] Univ Fed Rio de Janeiro, Sch Dent, Dept Periodontol, Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro, Inst Microbiol, Dept Immunol, Rio De Janeiro, Brazil
[3] Natl Canc Inst, Div Expt Med, Rio De Janeiro, Brazil
[4] Univ Fed Rio de Janeiro, Inst Microbiol, Dept Med Microbiol, Rio De Janeiro, Brazil
来源
ORAL MICROBIOLOGY AND IMMUNOLOGY | 2009年 / 24卷 / 02期
关键词
aggressive periodontitis; checkerboard; flow cytometry; oxidative burst; phagocytosis; polymorphonuclear neutrophils; subgingival microbiota; POLYMORPHONUCLEAR LEUKOCYTE PHAGOCYTOSIS; PORPHYROMONAS-GINGIVALIS; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; SUPEROXIDE PRODUCTION; SYSTEMIC-DISEASE; BACTERIA; CHEMILUMINESCENCE; DEGRADATION; PREVALENCE; PMNS;
D O I
10.1111/j.1399-302X.2008.00484.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Polymorphonuclear neutrophil (PMN) dysfunctions have been associated with severe forms of periodontitis. This study evaluated the correlation between PMN phagocytosis and oxidative burst with the subgingival microbiota of patients with generalized aggressive periodontitis (GAgP). Heparinized peripheral blood samples were obtained from 18 GAgP patients and 11 periodontally healthy (PH) subjects, and PMNs were isolated on a Ficoll-Hypaque gradient. For phagocytosis analysis, PMNs were incubated with fluorescein-labeled Staphylococcus aureus. The oxidative burst was evaluated by incubation of PMNs with dihydroethidium and activation by S. aureus. The assays were examined using flow cytometry. Subgingival biofilm samples were obtained from periodontal sites with and without periodontitis and 24 species were detected by checkerboard. A significantly lower phagocytosis rate was observed for patients with GAgP compared with PH subjects over time (P < 0.05). No differences between groups were found for superoxide production. GAgP patients presented significantly higher prevalence and levels of Porphyromonas gingivalis, Tannerella forsythia, and Aggregatibacter actinomycetemcomitans serotype b than controls (P < 0.05). Significant negative correlations between T. forsythia and P. gingivalis and PMN functions were observed. GAgP subjects presented diminished phagocytic activity of peripheral PMNs and high prevalence and levels of classical periodontal pathogens.
引用
收藏
页码:124 / 132
页数:9
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