Bidirectional role of orphan nuclear receptor RORα in clock gene transcriptions demonstrated by a novel reporter assay system

被引：62

作者：

Nakajima, Y ^{[1
]}

Ikeda, M

Kimura, T

Honma, S

Ohmiya, Y

Honma, K

机构：

[1] AIST, Res Inst Cell Engn, Cell Dynam Res Grp, Ikeda, Osaka 5638577, Japan

[2] Saitama Med Sch, Res Ctr Genom Med, Mol Clock Project, Hidaka, Saitama 3501241, Japan

[3] Saitama Med Sch, Dept Physiol, Moroyama, Saitama 3500495, Japan

[4] Hokkaido Univ, Grad Sch Med, Dept Physiol, Sapporo, Hokkaido 0608638, Japan

来源：

FEBS LETTERS | 2004年 / 565卷 / 1-3期

关键词：

circadian clock; reporter assays; green and red-emitting luciferases; ROR alpha; BMAL1;

D O I：

10.1016/j.febslet.2004.03.083

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Circadian rhythms are generated by an extremely complicated transcription-translation feedback loop. To precisely analyze the molecular mechanisms of the circadian clock, it is critical to monitor multiple gene expressions and/or interactions with their transcription factors simultaneously. We have developed a novel reporter assay system, the tricolor reporter in vitro assay system, which consists of green- and red-emitting Phrixothrix luciferases as dual reporters and blue-emitting Renilla luciferase as internal control. We have successfully employed this system in analyzing the effects of clock gene products on the enhancer elements of Bmal1 and Per1 promoters. The results indicate that the orphan nuclear receptor RORalpha regulates bidirectionally Bmal1 (positively) and Per1 (negatively) transcriptions simultaneously. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：122 / 126

页数：5

共 20 条

[11] Circadian rhythms from flies to human [J].

Panda, S ;

Hogenesch, JB ;

Kay, SA .

NATURE, 2002, 417 (6886) :329-335

[12] The orphan nuclear receptor REV-ERBα controls circadian transcription within the positive limb of the mammalian circadian oscillator [J].

Preitner, N ;

Damiola, F ;

Molina, LL ;

Zakany, J ;

Duboule, D ;

Albrecht, U ;

Schibler, U .

CELL, 2002, 110 (02) :251-260

[13] TRANSPLANTED SUPRACHIASMATIC NUCLEUS DETERMINES CIRCADIAN PERIOD [J].

RALPH, MR ;

FOSTER, RG ;

DAVIS, FC ;

MENAKER, M .

SCIENCE, 1990, 247 (4945) :975-978

[14] Coordination of circadian timing in mammals [J].

Reppert, SM ;

Weaver, DR .

NATURE, 2002, 418 (6901) :935-941

[15] IDENTIFICATION OF RVR, A NOVEL ORPHAN NUCLEAR RECEPTOR THAT ACTS AS A NEGATIVE TRANSCRIPTIONAL REGULATOR [J].

RETNAKARAN, R ;

FLOCK, G ;

GIGUERE, V .

MOLECULAR ENDOCRINOLOGY, 1994, 8 (09) :1234-1244

[16]

RUSKA B, 1979, PHYSIOL REV, V59, P449

[17] A transcription factor response element for gene expression during circadian night [J].

Ueda, HR ;

Chen, WB ;

Adachi, A ;

Wakamatsu, H ;

Hayashi, S ;

Takasugi, T ;

Nagano, M ;

Nakahama, K ;

Suzuki, Y ;

Sugano, S ;

Iino, M ;

Shigeyoshi, Y ;

Hashimoto, S .

NATURE, 2002, 418 (6897) :534-539

[18] Thr226 is a key residue for bioluminescence spectra determination in beetle luciferases [J].

Viviani, V ;

Uchida, A ;

Suenaga, N ;

Ryufuku, M ;

Ohmiya, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 280 (05) :1286-1291

[19] Cloning, sequence analysis, and expression of active Phrixothrix railroad-worms luciferases:: Relationship between bioluminescence spectra and primary structures [J].

Viviani, VR ;

Bechara, EJH ;

Ohmiya, Y .

BIOCHEMISTRY, 1999, 38 (26) :8271-8279

[20] Interactivating feedback loops within the mammalian clock: BMAL1 is negatively autoregulated and upregulated by CRY1, CRY2, and PER2 [J].

Yu, WJ ;

Nomura, M ;

Ikeda, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 290 (03) :933-941

← 1 2 →