Evaluation of a GFP reporter gene construct for environmental arsenic detection

被引:81
作者
Roberto, FF [1 ]
Barnes, JM [1 ]
Bruhn, DF [1 ]
机构
[1] EG&G Idaho Inc, Idaho Natl Engn Lab, Dept Biotechnol, Idaho Falls, ID 83415 USA
关键词
arsenate; arsenite; biosensor; reporter gene; lux; GFP;
D O I
10.1016/S0039-9140(02)00266-7
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Detection of arsenic and other heavy metal contaminants in the environment is critical to ensuring safe drinking water and effective cleanup of historic activities that have led to widespread contamination of soil and groundwater. Biosensors have the potential to significantly reduce the costs associated with site characterization and long term environmental monitoring. By exploiting the highly selective and sensitive natural mechanisms by which bacteria and other living organisms respond to heavy metals, and fusing transcriptionally active components of these mechanisms to reporter genes, such as P-galactosidase, bacterial luciferase (lux), or green fluorescent protein (GFP) from marine jellyfish, it is possible to produce inexpensive, yet effective biosensors. This article describes the response to submicrogram quantities of arsenite and arsenate of a whole cell arsenic biosensor utilizing a GFP reporter gene. Published by Elsevier Science B.V.
引用
收藏
页码:181 / 188
页数:8
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