Xanthine oxidase modified glassy carbon paste electrode

被引：53

作者：

Anik, U ^{[1
]}

Timur, S

Wang, J

Telefoncu, A

机构：

[1] Ege Univ, Fac Sci, Dept Chem, TR-35100 Izmir, Turkey

[2] Ege Univ, Fac Sci, Dept Biochem, TR-35100 Izmir, Turkey

[3] New Mexico State Univ, Dept Chem & Biochem, Las Cruces, NM 88003 USA

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2004年 / 6卷 / 09期

关键词：

glassy carbon paste electrode; biosensor; xanthine oxidase; xanthine; hypoxanthine;

D O I：

10.1016/j.elecom.2004.07.001

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 [应用化学];

摘要：

Glassy carbon paste electrode based on xanthine oxidase (XO) was developed for the determination of xanthine (X). The biosensor employs the amperometric detection of hydrogen peroxide evaluated by the enzymatic reaction catalyzed by XO immobilizing in the glassy carbon paste material. The optimum operational conditions for the biosensor were investigated and the system was calibrated for both hypoxanthine and xanthine, respectively. A linearity for the biosensor was obtained in concentration range between 5.0 x 10(-7) - 4.0 x 10(-5) M xanthine and 2.0 x 10(-5) - 8.0 x 10(-5) M hypoxanthine with detection limits of 1.0 x 10(-7) and 5.3 x 10(-6) M, respectively. Furthermore, as well as optimization of enzyme amount, sample application and accuracy were also searched. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：913 / 916

页数：4

共 13 条

[1]

[Anonymous], ELECTROANALYTICAL CH

[2]

Effect of surface activation on charge and mass transfer rates of the hexacyanoferrate(III)/(II) redox probe at fibrinogen-modified carbon paste electrodes [J].