Human histone deacetylase SIRT2 interacts with the homeobox transcription factor HOXA10

被引:70
作者
Bae, NS
Swanson, MJ
Vassilev, A
Howard, BH
机构
[1] NICHHD, Lab Mol & Growth Regulat, NIH, Bethesda, MD 20892 USA
[2] NICHHD, Lab Eukaryot Gene Regulat, NIH, Bethesda, MD 20892 USA
关键词
HOXA10; SIRT2;
D O I
10.1093/jb/mvh084
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Histone deacetylases are required for transcriptional repression in eukaryotes. Saccharomyces cerevisiae has several histone deacetylases, of which ySir2p is the most conserved throughout evolution. Currently, there is no report on the interacting protein partner of a human Sir2 homolog, SIRT2. Here we show for the first time that SIRT2 interacts with the homeobox transcription factor, HOXA10, which was identified in a two-hybrid screen. Interactions were confirmed by co-immunoprecipitation from in vitro translations as well as in human cell-free extracts. Taken together with mouse knockout studies, our results raise the intriguing possibility that SIRT2 plays a role in mammalian development.
引用
收藏
页码:695 / 700
页数:6
相关论文
共 30 条
[1]   Characterization of a human gene with sequence homology to Saccharomyces cerevisiae SIR2 [J].
Afshar, G ;
Murnane, JP .
GENE, 1999, 234 (01) :161-168
[2]   Histone modifications in transcriptional regulation [J].
Berger, SL .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 2002, 12 (02) :142-148
[3]   p21 is a transcriptional target of HOXA10 in differentiating myelomonocytic cells [J].
Bromleigh, VC ;
Freedman, LP .
GENES & DEVELOPMENT, 2000, 14 (20) :2581-2586
[4]  
Capecchi MR, 1997, COLD SPRING HARB SYM, V62, P273, DOI 10.1101/SQB.1997.062.01.034
[5]  
Chang CP, 1996, MOL CELL BIOL, V16, P1734
[6]   Role for human SIRT2 NAD-dependent deacetylase activity in control of mitotic exit in the cell cycle [J].
Dryden, SC ;
Nahhas, FA ;
Nowak, JE ;
Goustin, AS ;
Tainsky, MA .
MOLECULAR AND CELLULAR BIOLOGY, 2003, 23 (09) :3173-3185
[7]   Tyrosine phosphorylation of HoxA10 decreases DNA binding and transcriptional repression during interferon γ-induced differentiation of myeloid leukemia cell lines [J].
Eklund, EA ;
Jalava, A ;
Kakar, R .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (26) :20117-20126
[8]   Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins [J].
Frye, RA .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 273 (02) :793-798
[9]   Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity [J].
Frye, RA .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 260 (01) :273-279
[10]   Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase [J].
Imai, S ;
Armstrong, CM ;
Kaeberlein, M ;
Guarente, L .
NATURE, 2000, 403 (6771) :795-800