DNA display I. Sequence-encoded routing of DNA populations

被引：132

作者：

Halpin, DR ^{[1
]}

Harbury, PB ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA

来源：

PLOS BIOLOGY | 2004年 / 2卷 / 07期

关键词：

D O I：

10.1371/journal.pbio.0020173

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently reported technologies for DNA-directed organic synthesis and for DNA computing rely on routing DNA populations through complex networks. The reduction of these ideas to practice has been limited by a lack of practical experimental tools. Here we describe a modular design for DNA routing genes, and routing machinery made from oligonuclecitides and commercially available chromatography resins. The routing machinery partitions nanomole quantities of DNA into physically distinct subpools based on sequence. Partitioning steps can be iterated indefinitely, with worst-case yields of 85% per step. These techniques facilitate DNA-programmed chemical synthesis, and thus enable a materials biology that could revolutionize drug discovery.

引用

页码：1015 / 1021

页数：7

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