P120 catenin is required for morphogenetic movements involved in the formation of the eyes and the cranlofacial skeleton in Xenopus

被引:43
作者
Ciesiolka, M
Delvaeye, M
Van Imschoot, G
Verschuere, V
McCrea, P
van Roy, F
Vleminckx, K
机构
[1] Univ Ghent, Dev Biol Unit, B-9052 Ghent, Belgium
[2] Ghent Univ VIB, Mol Cell Biol Unit, Dept Mol Biomed Res, B-9052 Ghent, Belgium
[3] Univ Texas, MD Anderson Canc Ctr, Dept Biochem & Mol Biol, Programs Genet & Dev, Houston, TX 77030 USA
关键词
embryogenesis; cadherins; catenins; rho GTPases; xenopus;
D O I
10.1242/jcs.01298
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
During Xenopus development, p120 transcripts are enriched in highly morphogenetic tissues. We addressed the developmental function of p120 by knockdown experiments and by expressing E-cadherin mutants unable to bind p120. This resulted in defective eye formation and provoked malformations in the craniofacial cartilage structures, derivatives of the cranial neural crest cells. Closer inspection showed that p120 depletion impaired evagination of the optic vesicles and migration of cranial neural crest cells from the neural tube into the branchial arches. These morphogenetic processes were also affected by p120-uncoupled cadherins or E-cadherin containing a deletion of the juxtamembrane domain. Irrespective of the manipulation that caused the malformations, coexpression of dominant-negative forms of either Rac1 or LIM kinase rescued the phenotypes. Wild-type RhoA and constitutively active Rho kinase caused partial rescue. Our results indicate that, in contrast to invertebrates, p120 is an essential factor for vertebrate development and an adequate balance between cadherin activity and cytoskeletal condition is critical for correct morphogenetic movements.
引用
收藏
页码:4325 / 4339
页数:15
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