Mutagenicity of the mycotoxin alternariol in cultured mammalian cells

被引:159
作者
Brugger, Eva-Maria
Wagner, Joerg
Schumacher, David M.
Koch, Karen
Podlech, Joachim
Metzler, Manfred
Lehmann, Leane
机构
[1] Univ Karlsruhe, Sect Food Chem & Toxicol, Inst Appl Biosci, D-76131 Karlsruhe, Germany
[2] Univ Karlsruhe, Inst Organ Chem, D-76131 Karlsruhe, Germany
关键词
alternariol; gene mutations; V79; cells; L5178Y tk(+/-) cells; TK gene; HPRT gene;
D O I
10.1016/j.toxlet.2006.01.001
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
The mycotoxin alternariol (AOH) is an important contaminant of fruit and cereal products. Concern about exposure to low levels of AOH was raised after the disclosure that contamination of food with the AOH-producing species Alternaria alternata is associated with oesophagal cancer. Previously we have reported that AOH induces kinetochore-negative micronuclei in cultured Chinese hamster V79 cells. The present-study investigates the mutagenicity of AOH at the hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene locus in V79 cells and at the thymidine kinase (TK) gene locus in mouse lymphoma L5178Y tk(+/-) cells (MLC). Concentrations of 10 mu M AOH and more gave rise to a significant and concentration-dependent induction of HPRT and TK mutations in V79 cells and in MLC, respectively. The mutagenic potency of AOH was about 50-fold lower than that of the established mutagen 4-nitroquinoline-N-oxide in both cell lines. Discrimination between small and large colonies in the TK assay revealed the predominant induction of small colonies, which are indicative for extensive chromosomal deletions and which correlated with the induction of micronuclei in MLC. The mutagenicity of AOH may have a bearing on the carcinogenicity of this mycotoxin. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:221 / 230
页数:10
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