KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems

被引：88

作者：

Takahashi, Junko ^{[1
]}

Rudsander, Ulla J. ^{[2
]}

Hedenstrom, Mattias ^{[3
]}

Banasiak, Alicja ^{[1
]}

Harholt, Jesper ^{[5
]}

Amelot, Nicolas ^{[1
]}

Immerzeel, Peter ^{[1
]}

Ryden, Peter ^{[7
]}

Endo, Satoshi ^{[1
]}

Ibatullin, Farid M. ^{[2
]}

Brumer, Harry ^{[2
]}

del Campillo, Elena ^{[6
]}

Master, Emma R. ^{[4
]}

Scheller, Henrik Vibe ^{[5
]}

Sundberg, Bjorn ^{[1
]}

Teeri, Tuula T. ^{[2
]}

Mellerowicz, Ewa J. ^{[1
]}

机构：

[1] SLU, Dept Forest Genet & Plant Physiol, Umea Plant Sci Ctr, S-90183 Umea, Sweden

[2] AlbaNova Univ Ctr, Swedish Ctr Biomimet Fiber Engn, Sch Biotechnol, Royal Inst Technol KTH, S-10691 Stockholm, Sweden

[3] Umea Univ, Dept Chem, S-90187 Umea, Sweden

[4] Univ Toronto, Dept Chem Engn & Appl Chem, Toronto, ON M5S 3E5, Canada

[5] Univ Copenhagen, Dept Plant Biol, Plant Mol Biol Lab, Fac Life Sci, DK-1871 Frederiksberg, Denmark

[6] Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA

[7] Inst Food Res, Dept Food Mat Sci, Norwich NR4 7UA, Norfolk, England

来源：

PLANT AND CELL PHYSIOLOGY | 2009年 / 50卷 / 06期

基金：

瑞典研究理事会;

关键词：

Aspen; Cellulase; Cellulose; Cell wall; Populus; Wood formation; CARBOHYDRATE-ACTIVE ENZYMES; SECONDARY CELL-WALL; HIGHER-PLANTS; C-13; NMR; WOOD FORMATION; BIOSYNTHESIS; EXPRESSION; THALIANA; ENDO-1,4-BETA-GLUCANASE; GENES;

D O I：

10.1093/pcp/pcp062

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

KORRIGAN1 (KOR1) is a membrane-bound cellulase implicated in cellulose biosynthesis. PttCel9A1 from hybrid aspen (Populus tremula L. tremuloides Michx.) has high sequence similarity to KOR1 and we demonstrate here that it complements kor1-1 mutants, indicating that it is a KOR1 ortholog. We investigated the function of PttCel9A1/KOR1 in Arabidopsis secondary growth using transgenic lines expressing 35S::PttCel9A1 and the KOR1 mutant line irx2-2. The presence of elevated levels of PttCel9A1/KOR1 in secondary walls of 35S::PttCel9A1 lines was confirmed by in muro visualization of cellulase activity. Compared with the wild type, 35S::PttCel9A1 lines had higher trifluoroacetic acid (TFA)-hydrolyzable glucan contents, similar Updegraff cellulose contents and lower cellulose crystallinity indices, as determined by C-13 solid-state nuclear magnetic resonance (NMR) spectroscopy. irx2-2 mutants had wild-type TFA-hydrolyzable glucan contents, but reduced Updegraff cellulose contents and higher than wild-type cellulose crystallinity indices. The data support the hypothesis that PttCel9A1/KOR1 activity is present in cell walls, where it facilitates cellulose biosynthesis in a way that increases the amount of non-crystalline cellulose.

引用

页码：1099 / 1115

页数：17

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