First Report of Pseudobodo sp, a New Pathogen for a Potential Energy-Producing Algae: Chlorella vulgaris Cultures

被引：25

作者：

Chen, Zhangran ^{[1
,2
,3
]}

Lei, Xueqian ^{[1
,2
]}

Zhang, Bangzhou ^{[1
,2
]}

Yang, Luxi ^{[1
,2
]}

Zhang, Huajun ^{[1
,2
]}

Zhang, Jingyan ^{[1
,2
]}

Li, Yi ^{[1
,2
]}

Zheng, Wei ^{[1
,2
]}

Tian, Yun ^{[1
,2
]}

Liu, Jingwen ^{[4
]}

Zheng, Tianling ^{[1
,2
,3
]}

机构：

[1] Xiamen Univ, Sch Life Sci, State Key Lab Marine Environm Sci, Xiamen, Peoples R China

[2] Xiamen Univ, Sch Life Sci, Minist Educ Coastal & Wetland Ecosyst, Key Lab, Xiamen, Peoples R China

[3] Xiamen Univ, ShenZhen Res Inst, Shenzhen, Peoples R China

[4] Jimei Univ, Bioengn Coll, Xiamen, Peoples R China

来源：

PLOS ONE | 2014年 / 9卷 / 03期

关键词：

ALGICIDAL BACTERIUM; RED-TIDE; PARAMECIUM-BURSARIA; MARINE BACTERIUM; RNA; INFECTS; VIRUS; DINOFLAGELLATE; PREDATION; STRAIN;

D O I：

10.1371/journal.pone.0089571

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Chlorella vulgaris, is a kind of single-celled green algae, which could serve as a potential source of food and energy because of its photosynthetic efficiency. In our study, a pathogenic organism targeting C. vulgaris was discovered. The algae-lytic activity relates to a fraction from lysates of infected C. vulgaris that was blocked upon filtration through a 3 mm filter. 18S rRNA gene sequence analysis revealed that it shared 99.0% homology with the protist Pseudobodo tremulans. Scanning electron microscope analysis showed that Pseudobodo sp. KD51 cells were approximately 4-5 mm long, biflagellate with an anterior collar around the anterior part of the cell in unstressed feeding cells. Besides the initial host, Pseudobodo sp. KD51 could also kill other algae, indicating its relatively wide predatory spectrum. Heat stability, pH and salinity tolerance experiments were conducted to understand their effects on its predatory activities, and the results showed that Pseudobodo sp. KD51 was heat-sensitive, and pH and salinity tolerant.

引用

页数：11

共 66 条

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