Cyclic amplification of protein misfolding: application to prion-related disorders and beyond

被引:128
作者
Soto, C [1 ]
Saborio, GP [1 ]
Anderes, L [1 ]
机构
[1] Serono Int SA, Geneva, Switzerland
关键词
D O I
10.1016/S0166-2236(02)02195-1
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Diverse human disorders, including the majority of neurodegenerative diseases, are thought to arise from the misfolding and aggregation of protein. We have recently described a novel technology to amplify cyclically misfolded proteins in vitro. This procedure, named protein misfolding cyclic amplification (PMCA), is conceptually analogous to DNA amplification by PCR and has tremendous implications for research and diagnosis. The PMCA concept has been proved on the amplification of prions implicated in the pathogenesis of transmissible spongiform encephalopathies. In this article we describe the rational behind PMCA and some of the many potential applications of this novel technology.
引用
收藏
页码:390 / 394
页数:5
相关论文
共 37 条
[1]  
Aguzzi A, 2000, HAEMATOLOGICA, V85, P3
[2]   Blood infectivity and the prospects for a diagnostic screening test in Creutzfeldt-Jakob disease [J].
Brown, P ;
Cervenáková, L ;
Diringer, H .
JOURNAL OF LABORATORY AND CLINICAL MEDICINE, 2001, 137 (01) :5-13
[3]   MICE DEVOID OF PRP ARE RESISTANT TO SCRAPIE [J].
BUELER, H ;
AGUZZI, A ;
SAILER, A ;
GREINER, RA ;
AUTENRIED, P ;
AGUET, M ;
WEISSMANN, C .
CELL, 1993, 73 (07) :1339-1347
[4]   Conformational disease [J].
Carrell, RW ;
Lomas, DA .
LANCET, 1997, 350 (9071) :134-138
[5]   Scrapie infectivity correlates with converting activity, protease resistance, and aggregation of scrapie-associated prion protein in guanidine denaturation studies [J].
Caughey, B ;
Raymond, GJ ;
Kocisko, DA ;
Lansbury, PT .
JOURNAL OF VIROLOGY, 1997, 71 (05) :4107-4110
[6]   Inhibition of protease-resistant prion protein formation by porphyrins and phthalocyanines [J].
Caughey, WS ;
Raymond, LD ;
Horiuchi, M ;
Caughey, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (21) :12117-12122
[7]   Specific inhibition of in vitro formation of protease-resistant prion protein by synthetic peptides [J].
Chabry, J ;
Caughey, B ;
Chesebro, B .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (21) :13203-13207
[8]   Prion diseases - BSE and prions: Uncertainties about the agent [J].
Chesebro, B .
SCIENCE, 1998, 279 (5347) :42-43
[9]   Pathologic conformations of prion proteins [J].
Cohen, FE ;
Prusiner, SB .
ANNUAL REVIEW OF BIOCHEMISTRY, 1998, 67 :793-+
[10]   Protein misfolding, evolution and disease [J].
Dobson, CM .
TRENDS IN BIOCHEMICAL SCIENCES, 1999, 24 (09) :329-332