DNA Nanoflowers for Multiplexed Cellular Imaging and Traceable Targeted Drug Delivery

被引：377

作者：

Hu, Rong ^{[1
,2
]}

Zhang, Xiaobing ^{[1
,2
]}

Zhao, Zilong ^{[1
,2
]}

Zhu, Guizhi ^{[1
,2
]}

Chen, Tao ^{[1
,2
]}

Fu, Ting ^{[1
,2
]}

Tan, Weihong ^{[1
,2
]}

机构：

[1] Hunan Univ, Coll Biol, State Key Lab Chemo Biosensing & Chemometr, Mol Sci & Biomed Lab, Changsha 410082, Hunan, Peoples R China

[2] Hunan Univ, Coll Chem & Chem Engn, Collaborat Res Ctr Chem & Mol Med, Changsha 410082, Hunan, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2014年 / 53卷 / 23期

关键词：

aptamers; DNA nanoparticles; drug delivery; multicolor imaging; single-wavelength excitation; SILICA NANOPARTICLES; FLUORESCENT PROTEIN; LIVE CELLS; EXCITATION; RELEASE;

D O I：

10.1002/anie.201400323

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

We present a facile approach to make aptamer-conjugated FRET (fluorescent resonance energy transfer) nanoflowers (NFs) through rolling circle replication for multiplexed cellular imaging and traceable targeted drug delivery. The NFs can exhibit multi-fluorescence emissions by a single-wavelength excitation as a result of the DNA matrix covalently incorporated with three dye molecules able to perform FRET. Compared with the conventional DNA nanostructure assembly, NF assembly is independent of template sequences, avoiding the otherwise complicated design of DNA building blocks assembled into nanostructures by base-pairing. The NFs were uniform and exhibited high fluorescence intensity and excellent photostability. Combined with the ability of traceable targeted drug delivery, these colorful DNA NFs provide a novel system for applications in multiplex fluorescent cellular imaging, effective screening of drugs, and therapeutic protocol development.

引用

页码：5821 / 5826

页数：6

共 32 条

[1]

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