Synthesis of fluorescently labelled oligonucleotides and nucleic acids

被引：58

作者：

Davies, MJ ^{[1
]}

Shah, A ^{[1
]}

Bruce, IJ ^{[1
]}

机构：

[1] Univ Greenwich, Sch Chem & Life Sci, London SE18 6PF, England

来源：

CHEMICAL SOCIETY REVIEWS | 2000年 / 29卷 / 02期

关键词：

D O I：

10.1039/a905230e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The demand for and application of fluorescently labelled nucleic acid materials are growing, driven by ventures such as the Human Genome Mapping Project and the advent of DNA-based clinical diagnostics. This article surveys the strategies that are currently available for conjugation of fluorescent molecules to oligonucleotides and nucleic acids, and demonstrates how the labelled materials have been used in new and innovative assays for probing nucleic acid structure and properties.

引用

页码：97 / 107

页数：11

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