Single-base mutation detection using neutravidin-modified polystyrene nanoparticle aggregation

被引：31

作者：

Sato, K ^{[1
]}

Sawayanagi, M ^{[1
]}

Hosokawa, K ^{[1
]}

Maeda, M ^{[1
]}

机构：

[1] RIKEN, Inst Phys & Chem Res, Bioengn Lab, Wako, Saitama 3510198, Japan

来源：

ANALYTICAL SCIENCES | 2004年 / 20卷 / 06期

关键词：

D O I：

10.2116/analsci.20.893

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A single-base mutation assay using the non-crosslinking aggregation of neutravidin-modified polystyrene nanoparticles is described. This method requires only two steps: hybridization of biotinylated probe and sample DNAs, and then mixing with neutravidin-modified nanoparticles. The aggregation was detected within 20 min in total. A combination of the DNA non-crosslinking aggregation and biotin-avidin technology has allowed sufficient performance for the detection of single nucleotide polymorphisms.

引用

页码：893 / 894

页数：2

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