Temporal profile of ultrastructural changes in cortical neurons after a compression lesion

被引:5
作者
Andersson, B. [1 ]
Bjelke, B.
Sykova, E.
机构
[1] Acad Sci Czech Republ, Inst Expt Med, Videnska 1083, Prague 14020 4, Czech Republic
[2] Karolinska Inst, Dept Clin Neurosci, Stockholm, Sweden
[3] Charles Univ Prague, Fac Med 2, Dept Neurosci, Prague, Czech Republic
关键词
stroke; ischemia; sensory motor cortex; cell death; ultrastructure;
D O I
10.33549/physiolres.930723
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
We studied the occurrence of apoptosis and secondary delayed cell death at various time points in the penumbra zone, which is the target for therapeutic intervention after stroke. A compression lesion was induced in the right sensory motor cortex of rat brains. At 0.5, 1, 3, 6, 12, 24, 48 and 72 h after lesioning, motor functions were evaluated by behavioral tests, and cortical layers IV and V were examined by electron microscopy. Behavioral recovery was observed at 48 h after lesioning. At 0.5-1 h in the lesioned area, the neuropil was expanded and contained affected cells. Apoptotic cells were found between 0.5-72 h, and at 12 h, 47.3 % of the total cell number was apoptotic cells. On the contralateral side, cells showed an enlarged endoplasmic reticulum at 3 h, indicating secondary delayed cell death. Our results show that a compression lesion is a useful model for studying ultrastructural changes in injured cells. The lesion results in the penumbra zone with apoptotic cell death between 0.5-72 h. As secondary delayed cell death occurred on the contralateral side at three hours after lesioning might be the time period during which injured, but still viable, neurons can be targets for acute treatment.
引用
收藏
页码:339 / 348
页数:10
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