Improvement of evaporative light scattering detection of ceramides using triethylamine and formic acid in non-aqueous reversed phase liquid chromatography

被引：11

作者：

Gaudin, K ^{[1
]}

Chaminade, P ^{[1
]}

Ferrier, D ^{[1
]}

Baillet, A ^{[1
]}

机构：

[1] Univ Paris 11, Fac Pharm, Chim Analyt Lab, F-92290 Chatenay Malabry, France

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2000年 / 23卷 / 03期

关键词：

D O I：

10.1081/JLC-100101458

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of Porous Graphitized Carbon (PGC) stationary phase has enabled to emphasize the efficiency of the couple triethylamine (TEA) and formic acid (HCOOH) as a response enhancer for the Evaporative Light Scattering Detection (ELSD) of ceramides in Non-Aqueous Reversed Phase (NARP) Liquid Chromatography. 0.1% v/v of TEA and a stoechiometric amount of HCOOH greatly increase the intensity of the detection response whatever the nature of the eluent and the mobile phase composition was. This increase was not proportional to the amount of the TEA and HCOOH added as the response remained stable above 0.1 % v/v (7 mM).

引用

页码：387 / 397

页数：11

共 22 条

[1]

Cebolla V. L., 1997, Seminars in Food Analysis, V2, P171

[2] EVAPORATIVE ANALYZER AS A MASS DETECTOR FOR LIQUID-CHROMATOGRAPHY [J].

CHARLESWORTH, JM .

ANALYTICAL CHEMISTRY, 1978, 50 (11) :1414-1420

[3] SEPARATION OF LIPID CLASSES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH THE MASS DETECTOR [J].

CHRISTIE, WW .

JOURNAL OF CHROMATOGRAPHY, 1986, 361 :396-399

[4] Separation of several main glycolipids into classes and partially into species by HPLC and UV-detection [J].

Demopoulos, CA ;

Kyrili, M ;

Antonopoulou, S ;

Andrikopoulos, NK .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 1996, 19 (05) :771-781

[5] SEPARATION OF MOLECULAR-SPECIES OF CERAMIDES AS BENZOYL AND PARA-NITROBENZOYL DERIVATIVES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

DO, UH ;

PEI, PT ;

MINARD, RD .

LIPIDS, 1981, 16 (11) :855-862

[6] Simultaneous analysis of intact and desulfated glucosinolates with a porous graphitized carbon column [J].

Elfakir, C ;

Dreux, M .

JOURNAL OF CHROMATOGRAPHY A, 1996, 727 (01) :71-82

[7] Analysis of commercial ceramides by non-aqueous reversed-phase liquid chromatography with evaporative light-scattering detection [J].

Gaudin, K ;

Chaminade, P ;

Ferrier, D ;

Baillet, A ;

Tchapla, A .

CHROMATOGRAPHIA, 1999, 49 (5-6) :241-248

[8] Isolation of ceramide fractions from human stratum corneum lipid extracts by high-performance liquid chromatography [J].

Gildenast, T ;

Lasch, J .

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1997, 1346 (01) :69-74

[9] SEPARATION OF ANIONIC AND CATIONIC COMPOUNDS OF BIOMEDICAL INTEREST BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON POROUS GRAPHITIC CARBON [J].

GU, GH ;

LIM, CK .

JOURNAL OF CHROMATOGRAPHY, 1990, 515 :183-192

[10] INFLUENCE OF VARIOUS PARAMETERS ON THE RESPONSE FACTORS OF THE EVAPORATIVE LIGHT-SCATTERING DETECTOR FOR A NUMBER OF NON-VOLATILE COMPOUNDS [J].

GUIOCHON, G ;

MOYSAN, A ;

HOLLEY, C .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1988, 11 (12) :2547-2570

← 1 2 3 →