Very low amounts of glucose cause repression of the stress-responsive gene HSP12 in Saccharomyces cerevisiae

被引:22
作者
de Groot, E [1 ]
Bebelman, JP [1 ]
Mager, WH [1 ]
Planta, RJ [1 ]
机构
[1] Free Univ Amsterdam, Bioctr, Dept Biochem & Mol Biol, IMBW, NL-1081 HV Amsterdam, Netherlands
来源
MICROBIOLOGY-UK | 2000年 / 146卷
关键词
glucose repression; signal transduction; protein kinase A (PKA); glucose; 6-phosphate; HSP12;
D O I
10.1099/00221287-146-2-367
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Changing the growth mode of Saccharomyces cerevisiae by adding fermentable amounts of glucose to cells growing on a non-fermentable carbon source leads to rapid repression of general stress-responsive genes like HSP12, Remarkably, glucose repression of HSP12 appeared to occur even at very low glucose concentrations, down to 0.005%. Although these low levels of glucose do not induce fermentative growth, they do act as a growth signal, since upon addition of glucose to a concentration of 0.02%, growth rate increased and ribosomal protein gene transcription was up-regulated, In an attempt to elucidate how this type of glucose signalling may operate, several signalling mutants were examined, Consistent with the low amounts of glucose that elicit HSP12 repression, neither the main glucose-repression pathway nor cAMP-dependent activation of protein kinase A appeared to play a role in this regulation, Using mutants involved in glucose metabolism, evidence was obtained suggesting that glucose 6-phosphate serves as a signalling molecule, To identify the target for glucose repression on the promoter of the HSP12 gene, a promoter deletion series was used. The major transcription factors governing (stress-induced) transcriptional activation of HSP12 are Msn2p and Msn4p, binding to the general stress-responsive promoter elements (STREs). Surprisingly, glucose repression of HSP12 appeared to be independent of Msn2/4p: HSP12 transcription in glycerol-grown cells was unaffected in a Delta msn2 Delta msn4 strain, Nevertheless, evidence was obtained that STRE-mediated transcription is the target of repression by low amounts of glucose. These data suggest that an as yet unidentified factor is involved in STRE-mediated transcriptional regulation of HSP12.
引用
收藏
页码:367 / 375
页数:9
相关论文
共 47 条
[21]   Yeast pseudohyphal growth is regulated by GPA2, a G protein α homolog [J].
Lorenz, MC ;
Heitman, J .
EMBO JOURNAL, 1997, 16 (23) :7008-7018
[22]   IMPORTANCE OF A FLANKING AT-RICH REGION IN TARGET SITE RECOGNITION BY THE GC BOX-BINDING ZINC-FINGER PROTEIN MIG1 [J].
LUNDIN, M ;
NEHLIN, JO ;
RONNE, H .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (03) :1979-1985
[23]  
MAGER WH, 1991, MOL CELL BIOCHEM, V104, P181
[24]   The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress-response element (STRE) [J].
MartinezPastor, MT ;
Marchler, G ;
Schuller, C ;
MarchlerBauer, A ;
Ruis, H ;
Estruch, F .
EMBO JOURNAL, 1996, 15 (09) :2227-2235
[25]   Glucose repression in Saccharomyces cerevisiae is related to the glucose concentration rather than the glucose flux [J].
Meijer, MMC ;
Boonstra, J ;
Verkleij, AJ ;
Verrips, CT .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (37) :24102-24107
[26]  
Moskvina E, 1998, YEAST, V14, P1041, DOI 10.1002/(SICI)1097-0061(199808)14:11<1041::AID-YEA296>3.0.CO
[27]  
2-4
[28]   RESPONSE OF A YEAST GLYCOGEN-SYNTHASE GENE TO STRESS [J].
NI, HT ;
LAPORTE, DC .
MOLECULAR MICROBIOLOGY, 1995, 16 (06) :1197-1205
[29]   EUKARYOTIC RNA-POLYMERASE CONDITIONAL MUTANT THAT RAPIDLY CEASES MESSENGER-RNA SYNTHESIS [J].
NONET, M ;
SCAFE, C ;
SEXTON, J ;
YOUNG, R .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (05) :1602-1611
[30]  
Ozcan S, 1997, YEAST, V13, P127