Three-dimensional structure of the native spliceosome by cryo-electron microscopy

被引:55
作者
Azubel, M
Wolf, SG
Sperling, J
Sperling, R [1 ]
机构
[1] Hebrew Univ Jerusalem, Dept Genet, IL-91904 Jerusalem, Israel
[2] Weizmann Inst Sci, Dept Chem Res Support, IL-76100 Rehovot, Israel
[3] Weizmann Inst Sci, Dept Organ Chem, IL-76100 Rehovot, Israel
关键词
D O I
10.1016/j.molcel.2004.07.022
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Splicing of pre-mRNA occurs in a multicomponent macromolecular machine-the spliceosome. The spliceosome can be assembled in vitro by a stepwise assembly of a number of snRNPs and additional proteins on exogenously added pre-mRNA. In contrast, splicing in vivo occurs in preformed particles where endogenous pre-mRNAs are packaged with all five spliceosomal U snRNPs (penta-snRNP) together with other splicing factors. Here we present a three-dimensional image reconstruction by cryo-electron microscopy of native spliceosomes, derived from cell nuclei, at a resolution of 20 Angstrom. The structure revealed an elongated globular particle made up of two distinct subunits connected to each other leaving a tunnel in between. We show here that the larger subunit is a suitable candidate to accommodate the penta-snRNP, and that the tunnel could accommodate the pre-mRNA component of the spliceosome. The features this structure reveals provide new insight into the global architecture of the native splicing machine.
引用
收藏
页码:833 / 839
页数:7
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