Globins Synthesize the Second Messenger Bis-(3′-5′)-Cyclic Diguanosine Monophosphate in Bacteria

被引：70

作者：

Wan, Xuehua ^{[1
]}

Tuckerman, Jason R. ^{[2
]}

Saito, Jennifer A. ^{[1
]}

Freitas, Tracey Allen K. ^{[1
]}

Newhouse, James S. ^{[3
]}

Denery, Judith R. ^{[4
]}

Galperin, Michael Y. ^{[5
]}

Gonzalez, Gonzalo ^{[2
]}

Gilles-Gonzalez, Marie-Alda ^{[2
]}

Alam, Maqsudul ^{[1
,3
,6
]}

机构：

[1] Univ Hawaii, Dept Microbiol, Honolulu, HI 96822 USA

[2] Univ Texas SW Med Ctr Dallas, Dept Biochem, Dallas, TX 75390 USA

[3] Maui High Performance Comp Ctr, Maui, HI 96753 USA

[4] Univ Hawaii, Dept Mol Biosci & Bioengn, Honolulu, HI 96822 USA

[5] NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA

[6] Univ Hawaii, Adv Studies Genom Prote & Bioinformat, Honolulu, HI 96822 USA

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2009年 / 388卷 / 02期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

globin; oxygen sensor; c-di-GMP; diguanylate cyclase; biofilm; CYCLIC-DI-GMP; BORDETELLA-PERTUSSIS; DIGUANYLATE CYCLASE; ACETOBACTER-XYLINUM; CELLULOSE SYNTHESIS; ALLOSTERIC CONTROL; CRYSTAL-STRUCTURE; COUPLED SENSORS; MYOGLOBIN; RECEPTOR;

D O I：

10.1016/j.jmb.2009.03.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Globin-coupled sensors are heme-bolding signal transducers in Bacteria and Archaea in which an N-terminal globin controls the activity of a variable C-terminal domain. Here, we report that BpeGReg, a globin-coupled diguanylate cyclase from the whooping cough pathogen Bordetella pertussis, synthesizes the second messenger bis-(3'-5')-cyclic diguanosine monophosphate (c-di-GMP) upon oxygen binding. Expression of BpeGReg m Salmonella typhimurium enhances biofilm formation, while knockout of the BpeGReg gene of B. pertussis results in decreased biofilm formation. These results represent the first identification a signal ligand for any diguanylate cyclase and provide definitive experimental evidence that a globin-coupled sensor regulates c-di-GMP synthesis and biofilm formation. We propose that the synthesis of c-di-GMP by globin sensors is a widespread phenomenon in bacteria. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：262 / 270

页数：9

共 33 条

[1] New virulence-activated and virulence-repressed genes identified by systematic gene inactivation and generation of transcriptional fusions in Bordetella pertussis [J].