The formation of Cd-phytochelatin complexes in plant cell cultures

被引:42
作者
Kneer, R [1 ]
Zenk, MH [1 ]
机构
[1] UNIV MUNICH,LEHRSTUHL PHARMAZEUT BIOL,D-80333 MUNICH,GERMANY
关键词
Rauvolfia serpentina; Silene cucubalus; Cd-phytochelatin complexes; sulphide; formation and processing of Cd-phytochelatin complexes;
D O I
10.1016/S0031-9422(96)00514-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Three distinct Cd-phytochelatin complexes were purified to apparent homogeneity by gel filtration on Sephadex G50 of crude Cd-phytochelatin preparations isolated from Cd-treated suspension cultures of Rauvolfia serpentina. These purified complexes were analysed and found to be significantly different in PC chainlength, S:Cd ratio, and sulphide content. Kinetic measurements of PC, and sulphide content in Cd-treated suspension cultures of R. serpentina and Silene cucubalus showed that there are two possible ways of decreasing the amount of sulphur (and nitrogen) needed for Cd2+ sequestration: either by increasing the PC chainlength or by incorporating sulphide into the complexes. The latter process appears to be the more effective. Copyright (C) 1996 Elsevier Science Ltd
引用
收藏
页码:69 / 74
页数:6
相关论文
共 31 条
[1]   A GEL-ELECTROPHORESIS ASSAY FOR PHYTOCHELATINS [J].
ABRAHAMSON, SL ;
SPEISER, DM ;
OW, DW .
ANALYTICAL BIOCHEMISTRY, 1992, 200 (02) :239-243
[2]  
DAMERON CT, 1989, J BIOL CHEM, V264, P17355
[3]   CORRELATION BETWEEN THE DNA SUPERCOILING AND THE INITIATION OF TRANSCRIPTION BY ESCHERICHIA-COLI RNA-POLYMERASE INVITRO - ROLE OF THE SEQUENCES UPSTREAM OF THE PROMOTER REGION [J].
MISHRA, RK ;
GOPAL, V ;
CHATTERJI, D .
FEBS LETTERS, 1990, 260 (02) :273-276
[4]   TISSUE SULFHYDRYL GROUPS [J].
ELLMAN, GL .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1959, 82 (01) :70-77
[5]  
EVANS IM, 1990, FEBS LETT, V262, P29, DOI 10.1016/0014-5793(90)80145-9
[6]   CRYSTAL-STRUCTURE OF CD,ZN METALLOTHIONEIN [J].
FUREY, WF ;
ROBBINS, AH ;
CLANCY, LL ;
WINGE, DR ;
WANG, BC ;
STOUT, CD .
SCIENCE, 1986, 231 (4739) :704-710
[7]   INDUCTION OF HEAVY METAL-SEQUESTERING PHYTOCHELATIN BY CADMIUM IN CELL-CULTURES OF RAUVOLFIA-SERPENTINA [J].
GRILL, E ;
ZENK, MH ;
WINNACKER, EL .
NATURWISSENSCHAFTEN, 1985, 72 (08) :432-433
[8]   PHYTOCHELATINS - THE PRINCIPAL HEAVY-METAL COMPLEXING PEPTIDES OF HIGHER-PLANTS [J].
GRILL, E ;
WINNACKER, EL ;
ZENK, MH .
SCIENCE, 1985, 230 (4726) :674-676
[9]   PHYTOCHELATINS, THE HEAVY-METAL-BINDING PEPTIDES OF PLANTS, ARE SYNTHESIZED FROM GLUTATHIONE BY A SPECIFIC GAMMA-GLUTAMYLCYSTEINE DIPEPTIDYL TRANSPEPTIDASE (PHYTOCHELATIN SYNTHASE) [J].
GRILL, E ;
LOFFLER, S ;
WINNACKER, EL ;
ZENK, MH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (18) :6838-6842
[10]   SYNTHESIS OF 7 DIFFERENT HOMOLOGOUS PHYTOCHELATINS IN METAL-EXPOSED SCHIZOSACCHAROMYCES-POMBE CELLS [J].
GRILL, E ;
WINNACKER, EL ;
ZENK, MH .
FEBS LETTERS, 1986, 197 (1-2) :115-120