MicroRNA-145 regulates osteoblastic differentiation by targeting the transcription factor Cbfb

被引：67

作者：

Fukuda, Toru ^{[1
,7
]}

Ochi, Hiroki ^{[1
,7
]}

Sunamura, Satoko ^{[1
,7
]}

Haiden, Akina ^{[2
]}

Bando, Waka ^{[3
]}

Inose, Hiroyuki ^{[4
]}

Okawa, Atsushi ^{[5
,6
]}

Asou, Yoshinori ^{[5
,6
]}

Takeda, Shu ^{[1
,7
]}

机构：

[1] Tokyo Med & Dent Univ, Dept Physiol & Cell Biol, Bunkyo Ku, Tokyo 1138519, Japan

[2] Keio Univ, Sch Med, Dept Emergency & Crit Care Med, Shinjyuku Ku, Tokyo 1608582, Japan

[3] Keio Univ, Sch Med, Dept Internal Med, Sect Nephrol Endocrinol & Metab,Shinjyuku Ku, Tokyo 1608582, Japan

[4] Tokyo Med & Dent Univ, Dept Orthoped Surg, Bunkyo Ku, Tokyo 1138519, Japan

[5] Tokyo Med & Dent Univ, Int Res Ctr Mol Sci Tooth & Bone Dis, Dept Orthoped Surg, Bunkyo Ku, Tokyo 1138519, Japan

[6] Tokyo Med & Dent Univ, Int Res Ctr Mol Sci Tooth & Bone Dis, Global Ctr Excellence GCOE Program, Bunkyo Ku, Tokyo 1138519, Japan

[7] Japan Agcy Med Res & Dev, CREST, Chiyoda Ku, Tokyo 1000004, Japan

来源：

FEBS LETTERS | 2015年 / 589卷 / 21期

关键词：

MicroRNA; Osteoblast; Core binding factor beta; MESENCHYMAL STEM-CELLS; BINDING-FACTOR-BETA; BONE-FORMATION; OSTEOGENIC DIFFERENTIATION; SKELETAL DEVELOPMENT; LINEAGE PROGRESSION; ANIMAL DEVELOPMENT; RUNX2; MICE; MASS;

D O I：

10.1016/j.febslet.2015.09.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Osteoblastic differentiation is regulated by various factors, including hormones and transcription factors. Runt-related transcription factor 2 (Runx2) is an essential player in osteoblastogenesis and transactivates its molecular target by creating a protein complex with its hetero-dimeric partner core binding factor beta (Cbfb). However, the molecular regulation of Cbfb expression remains unknown. Here, we identified miR-145 as a crucial regulator of Cbfb expression. The expression of miR-145 increased during osteoblastogenesis, indicating that miR-145 works as an inhibitor of osteoblastogenesis. Stable expression of miR-145 decreased endogenous Cbfb expression and inhibited osteoblastogenesis, in cooperation with miR-34c. Furthermore, miR-145 decreased bone regeneration in vivo. Our results indicate that miR-145 physiologically regulates osteoblast differentiation and bone formation via Cbfb expression by forming a regulatory microRNA network. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3302 / 3308

页数：7

共 44 条

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