Atomic force microscopy: mechanical unfolding of proteins

被引：59

作者：

Rounsevell, R ^{[1
]}

Forman, JR ^{[1
]}

Clarke, J ^{[1
]}

机构：

[1] Univ Cambridge, Dept Chem, MRC Ctr Prot Engn, Cambridge CB2 1EW, England

来源：

METHODS | 2004年 / 34卷 / 01期

关键词：

D O I：

10.1016/j.ymeth.2004.03.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mechanical unfolding of proteins using atomic force microscopy is becoming a routine biophysical technique. Mechanistic investigations in this rapidly evolving field are beginning to resolve the factors that contribute to the behaviour of biological macromolecules under force. Here we describe the force-mode apparatus, the experimental set-up, tractable systems of study, and the analysis of the resultant force data. Finally we summarise some of the recent achievements and limitations of this technique. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：100 / 111

页数：12

共 58 条

[1] Pathways and intermediates in forced unfolding of spectrin repeats [J].

Altmann, SM ;

Grünberg, RG ;

Lenne, PF ;

Ylänne, J ;

Raae, A ;

Herbert, K ;

Saraste, M ;

Nilges, M ;

Hörber, JKH .

STRUCTURE, 2002, 10 (08) :1085-1096

[2]

BELL GI, 1978, SCIENCE, V200, P618, DOI 10.1126/science.347575

[3] Mechanical unfolding of a titin Ig domain: Structure of transition state revealed by combining atomic force microscopy, protein engineering and molecular dynamics simulations [J].

Best, RB ;

Fowler, SB ;

Herrera, JLT ;

Steward, A ;

Paci, E ;

Clarke, J .

JOURNAL OF MOLECULAR BIOLOGY, 2003, 330 (04) :867-877

[4] Force mode atomic force microscopy as a tool for protein folding studies [J].

Best, RB ;

Brockwell, DJ ;

Toca-Herrera, JL ;

Blake, AW ;

Smith, DA ;

Radford, SE ;

Clarke, J .

ANALYTICA CHIMICA ACTA, 2003, 479 (01) :87-105

[5] A simple method for probing the mechanical unfolding pathway of proteins in detail [J].

Best, RB ;

Fowler, SB ;

Toca-Herrera, JL ;

Clarke, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (19) :12143-12148

[6] Can non-mechanical proteins withstand force? Stretching barnase by atomic force microscopy and molecular dynamics simulation [J].

Best, RB ;

Li, B ;

Steward, A ;

Daggett, V ;

Clarke, J .

BIOPHYSICAL JOURNAL, 2001, 81 (04) :2344-2356

[7] Pulling geometry defines the mechanical resistance of a β-sheet protein [J].

Brockwell, DJ ;

Paci, E ;

Zinober, RC ;

Beddard, GS ;

Olmsted, PD ;

Smith, DA ;

Perham, RN ;

Radford, SE .

NATURE STRUCTURAL BIOLOGY, 2003, 10 (09) :731-737

[8] The effect of core destabilization on the mechanical resistance of I27 [J].

Brockwell, DJ ;

Beddard, GS ;

Clarkson, J ;

Zinober, RC ;

Blake, AW ;

Trinick, J ;

Olmsted, PD ;

Smith, DA ;

Radford, SE .

BIOPHYSICAL JOURNAL, 2002, 83 (01) :458-472

[9] ENTROPIC ELASTICITY OF LAMBDA-PHAGE DNA [J].

BUSTAMANTE, C ;

MARKO, JF ;

SIGGIA, ED ;

SMITH, S .

SCIENCE, 1994, 265 (5178) :1599-1600

[10] BIOCHEMICAL AND STRUCTURAL APPLICATIONS OF SCANNING FORCE MICROSCOPY [J].

BUSTAMANTE, C ;

ERIE, DA ;

KELLER, D .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1994, 4 (05) :750-760

← 1 2 3 4 5 6 →