Stem cell niche engineering through droplet microfluidics

被引：64

作者：

Allazetta, Simone ^{[1
,2
]}

Lutolf, Matthias P. ^{[1
,2
,3
]}

机构：

[1] Ecole Polytech Fed Lausanne, Sch Life Sci, Inst Bioengn, Lab Stem Cell Bioengn, CH-1015 Lausanne, Switzerland

[2] Ecole Polytech Fed Lausanne, Sch Engn, CH-1015 Lausanne, Switzerland

[3] Ecole Polytech Fed Lausanne, Sch Basic Sci, Inst Chem Sci & Engn, CH-1015 Lausanne, Switzerland

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2015年 / 35卷

基金：

瑞士国家科学基金会;

关键词：

MESENCHYMAL STROMAL CELLS; EXPANSION; MICROGELS; MICROCARRIERS; TECHNOLOGY; GENERATION; HYDROGELS; EMULSION; CULTURES; THERAPY;

D O I：

10.1016/j.copbio.2015.05.003

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Stem cells reside in complex niches in which their behaviour is tightly regulated by various biochemical and biophysical signals. In order to unveil some of the crucial stem cell-niche interactions and expedite the implementation of stem cells in clinical and pharmaceutical applications, in vitro methodologies are being developed to reconstruct key features of stem cell niches. Recently, droplet-based microfluidics has emerged as a promising strategy to build stem cell niche models in a miniaturized and highly precise fashion. This review highlights current advances in using droplet microfluidics in stem cell biology. We also discuss recent efforts in which microgel technology has been interfaced with high-throughput analyses to engender screening paradigms with an unparalleled potential for basic and applied biological studies.

引用

收藏

页码：86 / 93

页数：8

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