The β subunit of Aquifex aeolicus leucyl-tRNA synthetase is responsible for cognate tRNA recognition

被引：7

作者：

Gouda, M ^{[1
]}

Yokogawa, T ^{[1
]}

Nishikawa, K ^{[1
]}

机构：

[1] Gifu Univ, Fac Engn, Dept Biomol Sci, Gifu 5011193, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 297卷 / 04期

关键词：

Aquifex aeolicus; leucyl-tRNA synthetase; tRNA binding protein; heterodimer; quaternary structure;

D O I：

10.1016/S0006-291X(02)02281-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The active form of the leucyl-tRNA synthetase from an extreme thermophile Aquifex aeolicus has a heterodimeric (alpha/beta type) quaternary structure that is unique among class I aminoacyl-tRNA synthetases. In an attempt to clarify the individual roles of each subunit in the function of leucyl-tRNA synthetase, several elementary activities were separately measured using each of the subunits alone or the reconstructed alpha/beta complex. It was found that the P subunit alone is capable of recognizing its cognate tRNA, while the leucyl-adenylate formation and the overall leucyl-tRNA formation are detected only when both of the subunit proteins coexisted. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：950 / 955

页数：6

共 18 条

[1] Structural and functional considerations of the aminoacylation reaction [J].

Arnez, JG ;

Moras, D .

TRENDS IN BIOCHEMICAL SCIENCES, 1997, 22 (06) :211-216

[2] L-Arginine recognition by yeast arginyl-tRNA synthetase [J].

Cavarelli, J ;

Delagoutte, B ;

Eriani, G ;

Gangloff, J ;

Moras, D .

EMBO JOURNAL, 1998, 17 (18) :5438-5448

[3] High-level expression and single-step purification of leucyl-tRNA synthetase from Escherichia coli [J].

Chen, JF ;

Li, Y ;

Wang, ED ;

Wang, YL .

PROTEIN EXPRESSION AND PURIFICATION, 1999, 15 (01) :115-120

[4] The 2 Å crystal structure of leucyl-tRNA synthetase and its complex with a leucyl-adenylate analogue [J].

Cusack, S ;

Yaremchuk, A ;

Tukalo, M .

EMBO JOURNAL, 2000, 19 (10) :2351-2361

[5] The complete genome of the hyperthermophilic bacterium Aquifex aeolicus [J].

Deckert, G ;

Warren, PV ;

Gaasterland, T ;

Young, WG ;

Lenox, AL ;

Graham, DE ;

Overbeek, R ;

Snead, MA ;

Keller, M ;

Aujay, M ;

Huber, R ;

Feldman, RA ;

Short, JM ;

Olsen, GJ ;

Swanson, RV .

NATURE, 1998, 392 (6674) :353-358

[6] PARTITION OF TRANSFER-RNA SYNTHETASES INTO 2 CLASSES BASED ON MUTUALLY EXCLUSIVE SETS OF SEQUENCE MOTIFS [J].

ERIANI, G ;

DELARUE, M ;

POCH, O ;

GANGLOFF, J ;

MORAS, D .

NATURE, 1990, 347 (6289) :203-206

[7] Leucyl-tRNA synthetase from the extreme thermophile Aquifex aeolicus has a heterodimeric quaternary structure [J].

Gouda, M ;

Yokogawa, T ;

Asahara, H ;

Nishikawa, K .

FEBS LETTERS, 2002, 518 (1-3) :139-143

[8] In vitro selected RNA molecules that bind to elongation factor Tu [J].

Hornung, V ;

Hofmann, HP ;

Sprinzl, M .

BIOCHEMISTRY, 1998, 37 (20) :7260-7267

[9] A STRUCTURE-BASED MULTIPLE SEQUENCE ALIGNMENT OF ALL CLASS-I AMINOACYL-TRANSFER-RNA SYNTHETASES [J].

LANDES, C ;

PERONA, JJ ;

BRUNIE, S ;

ROULD, MA ;

ZELWER, C ;

STEITZ, TA ;

RISLER, JL .

BIOCHIMIE, 1995, 77 (03) :194-203

[10]

Martinis S A, 1997, Nucleic Acids Symp Ser, V36, P125

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