High-yield, solid-phase synthesis of humanin, an Alzheimer's disease associated, novel 24-mer peptide which contains a difficult sequence

被引：13

作者：

Evangelou, A ^{[1
]}

Zikos, C ^{[1
]}

Livaniou, E ^{[1
]}

Evangelatos, GP ^{[1
]}

机构：

[1] Natl Ctr Sci Res Demokritos, Immunopeptide Chem Lab, Inst Radioisotopes Radiodiagnost Prod, GR-15310 Athens, Greece

来源：

JOURNAL OF PEPTIDE SCIENCE | 2004年 / 10卷 / 10期

关键词：

Alzheimer's disease; difficult sequence; Fmoc-deprotection; humanin; solid-phase peptide synthesis; 2-Cl-tritylamidomethyl polystyrene resin;

D O I：

10.1002/psc.572

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Humanin is a novel, 24-mer residue bioactive peptide, which antagonizes Alzheimer's disease (AD) related neurotoxicity and offers a hope for developing new therapeutics against AD. Access to adequate amounts of pure humanin is a prerequisite for further, thorough, investigation of the pharmacological proper-ties and therapeutic potency of the peptide. Until now, humanin has been obtained mainly by molecular biology techniques. In this work the Fmoc solid-phase synthesis of humanin on an in-house prepared 2-Cl-tritylamidomethyl polystyrene resin is described fully. Special precautions, i.e. prolonged deprotection steps, should be taken to achieve a high overall yield, since humanin seems to contain a 'difficult sequence' (R(4)G(5)F(6)S(7)C(8)L(9)) near its highly lipophilic, biologically important region (LLLL12)-L-9-L-10-L-11. Copyright (C) 2004 European Peptide Society and John Wiley Sons, Ltd.

引用

页码：631 / 635

页数：5

共 19 条

[1] PREPARATION AND USE OF THE 4-[1-[N-(9-FLUORENYLMETHYLOXYCARBONYL)-AMINO]-2-(TRIMETHYLSILYL)ETHYL]PHENOXYACETIC ACID LINKAGE AGENT FOR SOLID-PHASE SYNTHESIS OF C-TERMINAL PEPTIDE AMIDES - IMPROVED YIELDS OF TRYPTOPHAN-CONTAINING PEPTIDES [J].