A facile transgalactosylation catalyzed by a lipid-coated beta-D-galactosidase in the water-organic two phases

被引：10

作者：

Mori, T ^{[1
]}

Fujita, S ^{[1
]}

Okahata, Y ^{[1
]}

机构：

[1] TOKYO INST TECHNOL, DEPT BIOMOL ENGN, MIDORI KU, YOKOHAMA, KANAGAWA 226, JAPAN

来源：

CHEMISTRY LETTERS | 1997年 / 01期

关键词：

D O I：

10.1246/cl.1997.73

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A lipid-coated beta-D-galactosidase could act as an efficient catalyst for transgalactosylation in the water-organic two phases: both the hydrophobic lipid-coated enzyme and alcohols were solubilized in isopropyl ether and mixed with an aqueous solution of lactose (a galactosyl donor). When a native beta-D-galactosidase was employed for the same reaction, neither the transgalactosylation nor the hydrolysis reaction proceeded due to the deactivation of the enzyme at the interface.

引用

页码：73 / 74

页数：2

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