Genetic characterization of Anaplasma (Ehrlichia) platys in dogs in Spain

被引:37
作者
Aguirre, E.
Tesouro, M. A.
Ruiz, L.
Amusateugui, I.
Sainz, A. [1 ]
机构
[1] Univ Complutense Madrid, Fac Vet, Dept Med & Cirugia Anim, E-28040 Madrid, Spain
[2] Univ Leon, Fac Vet, Dept Patol Anim, E-24071 Leon, Spain
[3] Clin Vet Garcia Noblejas, Madrid 28037, Spain
来源
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH | 2006年 / 53卷 / 04期
关键词
D O I
10.1111/j.1439-0450.2006.00937.x
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 [兽医学];
摘要
This paper reports the first genetic characterization of Ana-plasma (Ehrlichia) platys in Spain from a naturally infected dog. The dog presented clinical signs compatible with canine ehrlichiosis. After DNA extraction and PCR amplification, 16S rRNA gene and citrate synthase gene (gltA) of this agent were amplified. The GeriBank accession number for the nucleotide sequence of the 16S rRNA gene of this strain is AY530806. The A. platys strains registered in France and Japan showed the highest similarity to the 16S rRNA gene sequence obtained front the Spanish strain. In the amplification of the gltA gene, a 1443 bp fragment was obtained, and three nucleotide differences were detected in comparison with other strains sequences. These data confirm the presence of A. platys in a dog showing clinical signs compatible with ehrlichiosis in Spain.
引用
收藏
页码:197 / 200
页数:4
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