Quantitative phase tomography of Arabidopsis seeds reveals intercellular void network

被引：149

作者：

Cloetens, Peter

Mache, Regis

Schlenker, Michel

Lerbs-Mache, Silva

机构：

[1] Univ Grenoble 1, Lab Plastes & Differenciat Cellulaire, CNRS, F-38041 Grenoble 9, France

[2] European Synchrotron Radiat Facil, F-38043 Grenoble, France

[3] Univ Grenoble 1, Lab Louis Neel, CNRS, Inst Natl Polytech Grenoble, F-38042 Grenoble, France

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2006年 / 103卷 / 39期

关键词：

mature seed structure; synchrotron radiation; x-ray imaging; embryo organs; cell components;

D O I：

10.1073/pnas.0603490103

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We used quantitative phase tomography with synchrotron radiation to elucidate the 3D structure of Arabidopsis seeds in their native state. The cells are clearly distinguished, and their internal structure is revealed through local variations in electron density. We visualized a 3D network of intercellular air space that might allow immediate gas exchange for energy supply during germination and/or serve for rapid water uptake and distribution during imbibition.

引用

页码：14626 / 14630

页数：5

共 28 条

[1] Bewley J.D., 2013, SEEDS, DOI DOI 10.1007/978-1-4899-1002-8_1
[2] Seed germination and dormancy
Bewley, JD
[J]. PLANT CELL, 1997, 9 (07) : 1055 - 1066
[3] RESPIRATORY METABOLISM AND GENE-EXPRESSION DURING SEED-GERMINATION
BOTHA, FC
POTGIETER, GP
BOTHA, AM
[J]. PLANT GROWTH REGULATION, 1992, 11 (03) : 211 - 224
[4] Holotomography: Quantitative phase tomography with micrometer resolution using hard synchrotron radiation x rays
Cloetens, P
Ludwig, W
Baruchel, J
Van Dyck, D
Van Landuyt, J
Guigay, JP
Schlenker, M
[J]. APPLIED PHYSICS LETTERS, 1999, 75 (19) : 2912 - 2914
[5] Phase objects in synchrotron radiation hard x-ray imaging
Cloetens, P
Barrett, R
Baruchel, J
Guigay, JP
Schlenker, M
[J]. JOURNAL OF PHYSICS D-APPLIED PHYSICS, 1996, 29 (01) : 133 - 146
[6] PHASE RETRIEVAL THROUGH FOCUS VARIATION FOR ULTRA-RESOLUTION IN FIELD-EMISSION TRANSMISSION ELECTRON-MICROSCOPY
COENE, W
JANSSEN, G
DEBEECK, MO
VANDYCK, D
[J]. PHYSICAL REVIEW LETTERS, 1992, 69 (26) : 3743 - 3746
[7] Carbon dioxide diffusion inside leaves
Evans, JR
vonCaemmerer, S
[J]. PLANT PHYSIOLOGY, 1996, 110 (02) : 339 - 346
[8] Lignin distribution in wood cell walls determined by TEM and backscattered SEM techniques
Fromm, J
Rockel, B
Lautner, S
Windeisen, E
Wanner, G
[J]. JOURNAL OF STRUCTURAL BIOLOGY, 2003, 143 (01) : 77 - 84
[9] Variable timing of developmental progression in the stomatal pathway in Arabidopsis cotyledons
Geisler, M
Sack, FD
[J]. NEW PHYTOLOGIST, 2002, 153 (03) : 469 - 476
[10] HODDAD WS, 1994, SCIENCE, V2, P1213

← 1 2 3 →