A Lotus japonicus nodulation system based on heterologous expression of the fucosyl transferase NodZ and the acetyl transferase NolL in Rhizobium leguminosarum

被引：41

作者：

Bras, CP

Jordá, MA

Wijfjes, AHM

Harteveld, M

Stuurman, N

Thomas-Oates, JE

Spaink, HP

机构：

[1] Leiden State Univ, Inst Mol Plant Sci, NL-2333 AL Leiden, Netherlands

[2] UMIST, Michael Barber Ctr Mass Spectrometry, Manchester M60 1QD, Lancs, England

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2000年 / 13卷 / 04期

关键词：

acetyl fucose; mass spectrometry; Nod factors;

D O I：

10.1094/MPMI.2000.13.4.475

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Heterologous expression of NodZ and NoIL proteins in Rhizobium leguminosarum by. viciae led to the production of acetyl fucosylated lipo-chitin oligosaccharides (LCOs), indicating that the NoIL protein obtained from Mesorhizobium loti functions as an acetyl transferase. We show that the NoIL-dependent acetylation is specific for the fucosyl penta-N-acetylglucosamine species. In addition, the NoIL protein caused elevated production of LCOs, Efficient nodulation of Lotus japonicus by the NodZ/NoIL-producing strain was demonstrated. Nodulation efficiency was further improved by the addition of the ethylene inhibitor L-alpha-(2-aminoethoxyvinyl) glycine (AVG).

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收藏

页码：475 / 479

页数：5

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