siRNA-directed inhibition of HIV-1 infection

被引：626

作者：

Novina, CD ^{[1
]}

Murray, MF

Dykxhoorn, DM

Beresford, PJ

Riess, J

Lee, SK

Collman, RG

Lieberman, J

Shankar, P

Sharp, PA

机构：

[1] Harvard Univ, Sch Med, Ctr Blood Res, Boston, MA 02115 USA

[2] MIT, Ctr Canc Res, Cambridge, MA 02139 USA

[3] MIT, Dept Biol, Cambridge, MA USA

[4] MIT, McGovern Inst Brain Res, Cambridge, MA 02139 USA

[5] Harvard Univ, Sch Med, Dept Pediat, Boston, MA 02115 USA

[6] Univ Penn, Sch Med, Philadelphia, PA 19104 USA

来源：

NATURE MEDICINE | 2002年 / 8卷 / 07期

关键词：

D O I：

10.1038/nm725

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA interference silences gene expression through short interfering 21-23-mer double-strand RNA segments that guide mRNA degradation in a sequence-specific fashion. Here we report that siRNAs inhibit virus production by targeting the mRNAs for either the HIV-1 cellular receptor CD4, the viral structural Gag protein or green fluorescence protein substituted for the Nef regulatory protein. siRNAs effectively inhibit pre- and/or post-integration infection events in the HIV-1 life cycle. Thus, siRNAs may have potential for therapeutic intervention in HIV-1 and other viral infections.

引用

页码：681 / 686

页数：6

共 45 条

[21] Antisense RNA: Function and fate of duplex RNA in cells of higher eukaryotes
Kumar, M
Carmichael, GG
[J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (04) : 1415 - +
[22] Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells
Lee, NS
Dohjima, T
Bauer, G
Li, HT
Li, MJ
Ehsani, A
Salvaterra, P
Rossi, J
[J]. NATURE BIOTECHNOLOGY, 2002, 20 (05) : 500 - 505
[23] Frequency of CCR5 genotypes in HIV-infected patients in Roraima, Brazil
Guerra Corado, Andre de Lima
Villarouco da Silva, George Allan
Carvalho Leao, Renato Augusto
Granja, Fabiana
Naveca, Felipe Gomes
[J]. BRAZILIAN JOURNAL OF INFECTIOUS DISEASES, 2016, 20 (03) : 314 - 315
[24] THE T4 GENE ENCODES THE AIDS VIRUS RECEPTOR AND IS EXPRESSED IN THE IMMUNE-SYSTEM AND THE BRAIN
MADDON, PJ
DALGLEISH, AG
MCDOUGAL, JS
CLAPHAM, PR
WEISS, RA
AXEL, R
[J]. CELL, 1986, 47 (03) : 333 - 348
[25] HIV-1 STRUCTURAL GENE-EXPRESSION REQUIRES BINDING OF THE REV TRANS-ACTIVATOR TO ITS RNA TARGET SEQUENCE
MALIM, MH
TILEY, LS
MCCARN, DF
RUSCHE, JR
HAUBER, J
CULLEN, BR
[J]. CELL, 1990, 60 (04) : 675 - 683
[26] Gene silencing using micro-RNA designed hairpins
McManus, MT
Petersen, CP
Haines, BB
Chen, JZ
Sharp, PA
[J]. RNA, 2002, 8 (06) : 842 - 850
[27] Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells
Paddison, PJ
Caudy, AA
Bernstein, E
Hannon, GJ
Conklin, DS
[J]. GENES & DEVELOPMENT, 2002, 16 (08) : 948 - 958
[28] Use of a green fluorescent protein as a marker for human immunodeficiency virus type 1 infection
Page, KA
Liegler, T
Feinberg, MB
[J]. AIDS RESEARCH AND HUMAN RETROVIRUSES, 1997, 13 (13) : 1077 - 1081
[29] Cosuppression of nonhomologous transgenes in Drosophila involves mutually related endogenous sequences
Pal-Bhadra, M
Bhadra, U
Birchler, JA
[J]. CELL, 1999, 99 (01) : 35 - 46
[30] Effective expression of small interfering RNA in human cells
Paul, CP
Good, PD
Winer, I
Engelke, DR
[J]. NATURE BIOTECHNOLOGY, 2002, 20 (05) : 505 - 508

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