Isoreserpine promotes β-catenin degradation via Siah-1 up-regulation in HCT116 colon cancer cells

被引：25

作者：

Gwak, Jungsug ^{[1
]}

Song, Taeyun ^{[1
]}

Song, Jie-Young ^{[2
]}

Yun, Yeon-Sook ^{[2
]}

Choi, Il-whan ^{[3
]}

Jeong, Yongsu ^{[4
,5
]}

Shin, Jae-Gook ^{[1
,6
]}

Oh, Sangtaek ^{[1
]}

机构：

[1] Inje Univ, PharmacoGenom Res Ctr, Pusan 614735, South Korea

[2] Korea Inst Radiol & Med Sci, Lab Radiat Canc Sci, Seoul 139706, South Korea

[3] Inje Univ, Coll Med, Dept Microbiol, Ctr Viral Dis Res, Pusan 614735, South Korea

[4] Kyung Hee Univ, Dept Genet Engn, Yongin 446701, South Korea

[5] Kyung Hee Univ, Grad Sch Biotechnol, Yongin 446701, South Korea

[6] Inje Univ, Dept Clin Pharmacol, Busan Paik Hosp, Pusan 614735, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 387卷 / 03期

关键词：

Wnt/beta-catenin pathway; Isoreserpine; Colon cancer; Siah-1; Protein degradation; beta-Catenin; HUMAN HOMOLOG; TARGET; SUPPRESSION; ACTIVATION; PATHWAY; GROWTH; IDENTIFICATION; APOPTOSIS; COMPLEX; P53;

D O I：

10.1016/j.bbrc.2009.07.027

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Aberrant accumulation of intracellular beta-catenin in intestinal epithelial cells is a frequent early event during the development of colon cancer. To identify small molecules that decrease the level of intracellular beta-catenin, we performed cell-based chemical screening using genetically engineered HEK293 reporter cells to detect compounds that inhibit TOPFlash reporter activity, which was stimulated by Wnt3a-conditioned medium. We found that isoreserpine promoted the degradation of intracellular beta-catenin by upregulation of Siah-1 in HEK293 and HCT116 colon cancer cells. Moreover, isoreserpine repressed the expression of beta-catenin/T-cell factor (TCF)-dependent genes, Such as cyclin D1 and c-myc, resulting in the suppression of HCT116 cell proliferation. Our findings suggest that isoreserpine call potentially be used as a chemotherapeutic agent against colon cancer. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：444 / 449

页数：6

共 37 条

[1]

beta-catenin is a target for the ubiquitin-proteasome pathway [J].

Aberle, H ;

Bauer, A ;

Stappert, J ;

Kispert, A ;

Kemler, R .

EMBO JOURNAL, 1997, 16 (13) :3797-3804

[2]

HYPERTENSION TREATED WITH RAUWOLFIA SERPENTINA (WHOLE ROOT) AND WITH RESERPINE - CONTROLLED STUDY DISCLOSING OCCASIONAL SEVERE DEPRESSION [J].

ACHOR, RWP ;

HANSON, NO ;

GIFFORD, RW .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1955, 159 (09) :841-845

[3]

Isolation of 10 differentially expressed cDNAs in p53-induced apoptosis: Activation of the vertebrate homologue of the Drosophila seven in absentia gene [J].

Amson, RB ;

Nemani, M ;

Roperch, JP ;

Israeli, D ;

Bougueleret, L ;

LeGall, I ;

Medhioub, M ;

LinaresCruz, G ;

Lethrosne, F ;

Pasturaud, P ;

Piouffre, L ;

Prieur, S ;

Susini, L ;

Alvaro, V ;

Millasseau, P ;

Guidicelli, C ;

Bui, H ;

Massart, C ;

Cazes, L ;

Dufour, F ;

BruzzoniGiovanelli, H ;

Owadi, H ;

Hennion, C ;

Charpak, G ;

Dausset, J ;

Calvo, F ;

Oren, M ;

Cohen, D ;

Telerman, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (09) :3953-3957

[4]

Linking colorectal cancer to Wnt signaling [J].

Bienz, M ;

Clevers, H .

CELL, 2000, 103 (02) :311-320

[5]

Diclofenac attenuates Wnt/β-catenin signaling in colon cancer cells by activation of NF-κB [J].

Cho, M ;

Gwak, J ;

Park, S ;

Won, J ;

Kim, DE ;

Yea, SS ;

Cha, IJ ;

Kim, TK ;

Shin, JG ;

Oh, S .

FEBS LETTERS, 2005, 579 (20) :4213-4218

[6]

ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].

DIGNAM, JD ;

LEBOVITZ, RM ;

ROEDER, RG .

NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1475-1489

[7]

The ABC of APC [J].

Fearnhead, NS ;

Britton, MP ;

Bodmer, WF .

HUMAN MOLECULAR GENETICS, 2001, 10 (07) :721-733

[8]

Targeted suppression of β-catenin blocks intestinal adenoma formation in APC min mice [J].

Foley, Paul J. ;

Scheri, Randall P. ;

Smolock, Christopher J. ;

Pippin, James ;

Green, Douglas W. ;

Drebin, Jeffrey A. .

JOURNAL OF GASTROINTESTINAL SURGERY, 2008, 12 (08) :1452-1458

[9]

β-catenin antisense treatment decreases β-catenin expression and tumor growth rate in colon carcinoma xenografts [J].

Green, DW ;

Roh, H ;

Pippin, JA ;

Drebin, JA .

JOURNAL OF SURGICAL RESEARCH, 2001, 101 (01) :16-20

[10]

The F-box protein β-TrCP associates with phosphorylated β-catenin and regulates its activity in the cell [J].

Hart, M ;

Concordet, JP ;

Lassot, I ;

Albert, I ;

del los Santos, R ;

Durand, H ;

Perret, C ;

Rubinfeld, B ;

Margottin, F ;

Benarous, R ;

Polakis, P .

CURRENT BIOLOGY, 1999, 9 (04) :207-210

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