Isolation of genes related to abscisic acid production in Botrytis cinerea TB-3-H8 by cDNA-AFLP

被引:19
作者
Gong, Tao [1 ,2 ]
Shu, Dan [1 ]
Zhao, Ming [1 ,3 ]
Zhong, Juan [1 ]
Deng, Hong-Yuan [1 ,2 ]
Tan, Hong [1 ]
机构
[1] Chinese Acad Sci, Chengdu Inst Biol, Key Lab Environm & Appl Microbiol, Chengdu 610041, Peoples R China
[2] Univ Chinese Acad Sci, Beijing, Peoples R China
[3] Yunnan Agr Univ, Coll Pu Erh Tea, Kunming, Peoples R China
关键词
Botrytis cinerea; Abscisic acid (ABA); cDNA-amplified fragment length polymorphism (cDNA-AFLP); Real-time reverse transcription polymerase chain reaction (real-time PCR); STREPTOMYCES-CLAVULIGERUS; GIBBERELLIN BIOSYNTHESIS; TRANSCRIPT PROFILES; EXPRESSION; IDENTIFICATION; PATHWAY; CLONING; FUNGUS; PCR; DNA;
D O I
10.1002/jobm.201200311
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Abscisic acid (ABA) plays important roles in many aspects of plant growth and development. Botrytis cinerea TB-3-H8, a high-yield strain of ABA, was used to elucidate the molecular mechanisms of ABA production in the present work. cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique was applied to isolate genes differentially expressed between ABA high and low-yield conditions. This resulted in the identification of 856 differentially expressed transcript-derived fragments (TDFs). Forty-five TDFs that displayed obvious up-regulated expression profiles in the ABA high-yield condition were sequenced. Based on BlastX in NCBI, 31 TDFs were assumed to have homology with genes encoding proteins with known functions. According to molecular function of gene ontology (GO) analysis, the 31 TDFs were categorized to proteins with enzyme catalytic activities, transcription factor activities, transporter activities, and kinds of binding activities. Further confirmation of the differential expression of these sequences was carried out by performing semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) on 10 randomly selected TDFs. Five up-regulated genes were selected to analyze the expression profiles using real-time PCR. This study enriches our knowledge of the molecular basis for ABA biosynthesis in B. cinerea TB-3-H8.
引用
收藏
页码:204 / 214
页数:11
相关论文
共 56 条
[1]   Identification of genes expressed during early somatic embryogenesis in Pinus radiata [J].
Aquea, Felipe ;
Arce-Johnson, Patricio .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2008, 46 (5-6) :559-568
[2]   (+)-ABSCISIC ACID, A METABOLITE OF FUNGUS CERCOSPORA-ROSICOLA [J].
ASSANTE, G ;
MERLINI, L ;
NASINI, G .
EXPERIENTIA, 1977, 33 (12) :1556-1557
[3]   Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber development [J].
Bachem, CWB ;
vanderHoeven, RS ;
deBruijn, SM ;
Vreugdenhil, D ;
Zabeau, M ;
Visser, RGF .
PLANT JOURNAL, 1996, 9 (05) :745-753
[4]   FAST AND SENSITIVE SILVER STAINING OF DNA IN POLYACRYLAMIDE GELS [J].
BASSAM, BJ ;
CAETANOANOLLES, G ;
GRESSHOFF, PM .
ANALYTICAL BIOCHEMISTRY, 1991, 196 (01) :80-83
[5]   INTERMEDIATE STEPS IN THE BIOSYNTHESIS OF ABSCISIC-ACID FROM FARNESYL PYROPHOSPHATE IN CERCOSPORA-ROSICOLA [J].
BENNETT, RD ;
NORMAN, SM ;
MAIER, VP .
PHYTOCHEMISTRY, 1990, 29 (11) :3473-3477
[6]   A cDNA-AFLP approach to study ochratoxin A production in Aspergillus carbonarius [J].
Botton, Alessandro ;
Ferrigo, Davide ;
Scopel, Cristina ;
Causin, Roberto ;
Bonghi, Claudio ;
Ramina, Angelo .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2008, 127 (1-2) :105-115
[7]   REGULATION OF GIBBERELLIN BIOSYNTHESIS IN GIBBERELLA-FUJIKUROI [J].
CANDAU, R ;
AVALOS, J ;
CERDAOLMEDO, E .
PLANT PHYSIOLOGY, 1992, 100 (03) :1184-1188
[8]   cDNA AFLP-based techniques for studying transcript profiles in horses [J].
Cappelli, K ;
Porceddu, A ;
Verini-Supplizi, A ;
Capomaccio, S ;
De Marchis, F ;
Falcinelli, M ;
Gaiti, A ;
Silvestrelli, M .
RESEARCH IN VETERINARY SCIENCE, 2005, 79 (02) :105-112
[9]   Identification of new galactose oxidase genes in Fusarium spp. [J].
Cordeiro, Fabio Aparecido ;
Faria, Carla Bertechini ;
Barbosa-Tessmann, Ione Parra .
JOURNAL OF BASIC MICROBIOLOGY, 2010, 50 (06) :527-537
[10]   VIB-1 is required for expression of genes necessary for programmed cell death in Neurospora crassa [J].
Dementhon, Karine ;
Iyer, Gopal ;
Glass, N. Louise .
EUKARYOTIC CELL, 2006, 5 (12) :2161-2173