Double chip protein arrays using recombinant single-chain Fv antibody fragments

被引:12
作者
Gilbert, L
Schiffmann, S
Rubenwolf, S
Jensen, K
Mail, T
Albrecht, C
Lankenau, A
Beste, G
Blank, K
Gaub, HE
Clausen-Schaumann, H
机构
[1] Nanotype, Grafelfing, Germany
[2] LMV, Lehrstuhl Angew Phys, Munich, Germany
[3] LMV, Ctr NanoSci, Munich, Germany
关键词
multiplexing; protein array; recombinant antibody; sandwich immunoassay;
D O I
10.1002/pmic.200300736
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Protein arrays permit the parallel analysis of many different markers in a small sample volume. However, the problem of cross-reactivity limits the degree of multiplexing in parallel sandwich immunoassays (using monoclonal antibodies (mAbs)), meaning antibodies must be pre-screened in order to reduce false positives. In contrast, we use a second chip surface for the local application of detection antibodies, thereby efficiently eliminating antibody cross-reactions. Here, we illustrate the potential advantages of using single-chain Fv fragments rather than mAbs as capture and detection molecules with this double chip technology.
引用
收藏
页码:1417 / 1420
页数:4
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