multiplexing;
protein array;
recombinant antibody;
sandwich immunoassay;
D O I:
10.1002/pmic.200300736
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
Protein arrays permit the parallel analysis of many different markers in a small sample volume. However, the problem of cross-reactivity limits the degree of multiplexing in parallel sandwich immunoassays (using monoclonal antibodies (mAbs)), meaning antibodies must be pre-screened in order to reduce false positives. In contrast, we use a second chip surface for the local application of detection antibodies, thereby efficiently eliminating antibody cross-reactions. Here, we illustrate the potential advantages of using single-chain Fv fragments rather than mAbs as capture and detection molecules with this double chip technology.