The effect of DNA/gold particle preparation technique, and particle bombardment device, on the transformation of barley (Hordeum vulgare)

被引：26

作者：

Harwood, WA ^{[1
]}

Ross, SM ^{[1
]}

Cilento, P ^{[1
]}

Snape, JW ^{[1
]}

机构：

[1] John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England

来源：

EUPHYTICA | 2000年 / 111卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

barley; Hordeum vulgare; transformation; particle bombardment; particle gun;

D O I：

10.1023/A:1003700300235

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Immature embryos of the spring barley variety Golden Promise, were bombarded with three different particle delivery systems and both transient and stable transformation examined. In addition, a range of techniques for the preparation of the DNA coated gold particles was examined. Fertile transgenic barley plants were obtained using three particle preparation techniques which differed in the amount of gold and DNA used for each bombardment. However, only one of the particle delivery systems, the PDS 1000/He device, appeared to be effective in yielding transformed barley plants.

引用

页码：67 / 76

页数：10

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