Identification and characterization of a porcine monocytic cell line supporting porcine reproductive and respiratory syndrome virus (PRRSV) replication and progeny virion production by using an improved DNA-launched PRRSV reverse genetics system

被引：28

作者：

Huang, Y. W. ^{[1
]}

Fang, Y. ^{[2
]}

Meng, X. J. ^{[1
]}

机构：

[1] Virginia Polytech Inst & State Univ, Coll Vet Med, Dept Biomed Sci & Pathobiol, Ctr Mol Med & Infect Dis, Blacksburg, VA 24061 USA

[2] S Dakota State Univ, Dept Vet Sci, Brookings, SD 57007 USA

来源：

VIRUS RESEARCH | 2009年 / 145卷 / 01期

关键词：

Porcine reproductive and respiratory; syndrome virus (PRRSV); DNA-launched reverse genetics system; Ribozyme elements; Porcine monocytic cell line; INFECTIOUS CDNA-CLONE; FULL-LENGTH CDNA; ALVEOLAR MACROPHAGES; RNA VIRUS; NORTH-AMERICAN; UNITED-STATES; MOLECULAR-CLONING; CLINICAL-DISEASE; LYMPH-NODES; IN-VIVO;

D O I：

10.1016/j.virusres.2009.05.013

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In this study, an improved DNA-launched (plasmid DNA transfection-based) reverse genetics system with reduced cost and labor was developed for porcine reproductive and respiratory syndrome virus (PRRSV) by introduction of ribozyme elements at both termini of the viral genomic cDNA that were placed under the control of a eukaryotic hybrid promoter. The rescue efficacy of PRRSV with this system was approximately 10-50-fold higher than the in vitro-transcribed RNA-based system and the traditional DNA-launched system without the engineered ribozyme elements, as determined by reporter GFP level in transfected cells and the peak titer of the recovery virus. By using this new reverse genetics system, we identified and characterized a porcine monocytic cell line, 3D4/31, capable of supporting PRRSV replication, progeny virion production, and attachment on the cell surface. The establishment of this improved reverse genetic system and the identification of a porcine monocytic cell line supporting PRRSV replication will aid future studies of host-virus interaction of PRRSV. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1 / 8

页数：8

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