DNA triple helix stabilisation by covalent attachment of a tripler-specific ligand

被引：9

作者：

Keppler, MD

McKeen, CM

Zegrocka, O

Strekowski, L

Brown, T

Fox, KR

机构：

[1] Univ Southampton, Sch Biol Sci, Div Biochem & Mol Biol, Southampton SO16 7PX, Hants, England

[2] Oswel Res Prod Ltd, Lab 5005, Southampton SO16 7PX, Hants, England

[3] Georgia State Univ, Dept Chem, Atlanta, GA 30303 USA

[4] Univ Southampton, Dept Chem, Southampton SO17 1BJ, Hants, England

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1999年 / 1447卷 / 2-3期

关键词：

triplex ligand; footprinting; DNA triplex; naphthylquinoline;

D O I：

10.1016/S0167-4781(99)00114-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have prepared oligonucleotides with a naphthylquinoline tripler-binding ligand covalently tethered to the 5'-end and have used W-melting and DNase I footprinting to examine the stability of intra- and inter-molecular triplexes containing this modification. We find that covalent attachment of the ligand increases,the melting temperature of intramolecular 6-mer triplexes by about 14 K, and increases the binding of 9-mer oligonucleotides to their duplex target sites by about 60-fold. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：137 / 145

页数：9

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