An extended gate FET-based biosensor integrated with a Si microfluidic channel for detection of protein complexes

被引:80
作者
Kim, Dong-Sun
Park, Jee-Eun
Shin, Jang-Kyoo [1 ]
Kim, Pan Kyeom
Lim, Geunbae
Shoji, Shuichi
机构
[1] Kyungpook Natl Univ, Sch Elect & Elect Engn, Taegu 702701, South Korea
[2] Kyungpook Natl Univ, Dept Sensor Engn, Taegu 702701, South Korea
[3] Pohang Univ Sci & Technol, Dept Mech Engn, Pohang 790784, South Korea
[4] Waseda Univ, Dept Elect Engn & Biosci Engn, Tokyo, Japan
关键词
EGFET; microfluidic channel; FET-type biosensor; protein sensor; SPR; bio-AFM;
D O I
10.1016/j.snb.2006.01.018
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this work, we present an extended gate field effect transistor (EGFET)-based biosensor integrated with a silicon micro-fluidic channel for the electronic detection of streptavidin-biotin protein complexes. The connection between the EGFET and microfluidic system could be achieved with the proposed device, as it offers isolation between the device and solution, compatibility with the integrated circuit (IQ technology and, is applicable to the micro total analysis system (mu-TAS). The device was fabricated on the basis of semiconductor IC fabrication and micro-electro mechanical system (MEMS) technology. Au was used as the extended gate metal to form a self-assembled monolayer (SAM) with thiol. The bindings of the SAM, streptavidin and biotin were detected by measuring the electrical characteristics of the FET device. We also verified the interactions among the SAM, streptavidin, and biotin by using surface plasmon resonance (SPR) measurements. Furthermore, atomic force microscopy (AFM) images of the bio-layers formed on the Au electrode were taken in a solution in order to determine the presence of protein biomolecules with the proposed configuration. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:488 / 494
页数:7
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