Is it essential to use anhydrous acetonitrile in the manufacture of phosphorothioate oligonucleotides?

被引：21

作者：

Capaldi, DC ^{[1
]}

Scozzari, AN ^{[1
]}

Cole, DL ^{[1
]}

Ravikumar, VT ^{[1
]}

机构：

[1] ISIS Pharmaceut, Dev Chem, Carlsbad, CA 92008 USA

来源：

ORGANIC PROCESS RESEARCH & DEVELOPMENT | 1999年 / 3卷 / 06期

关键词：

D O I：

10.1021/op9900333

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

It is demonstrated that acetonitrile containing up to at least 200 ppm water can be used as the wash solvent and to prepare the capping and sulfur transfer reagent solutions used in large-scale solid-phase phosphorothioate oligonucleotide synthesis, thus replacing most of the expensive anhydrous acetonitrile previously used. No compromise in yield or product quality is observed, compared to a control synthesis performed using anhydrous acetonitrile throughout.

引用

页码：485 / 487

页数：3

共 14 条

[1] DEOXYNUCLEOSIDE PHOSPHORAMIDITES - A NEW CLASS OF KEY INTERMEDIATES FOR DEOXYPOLYNUCLEOTIDE SYNTHESIS [J].