Phenotypic conversion of drug-resistant bacteria to drug sensitivity

被引:66
作者
GuerrierTakada, C [1 ]
Salavati, R [1 ]
Altman, S [1 ]
机构
[1] YALE UNIV,DEPT BIOL,NEW HAVEN,CT 06520
关键词
bacterial drug resistance; external guide sequences; RNase P;
D O I
10.1073/pnas.94.16.8468
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Plasmids that contain synthetic genes coding for small oligoribonucleotides called external guide sequences (EGSs) have been introduced into strains of Escherichia coli harboring antibiotic resistance genes. The EGSs direct RNase P to cleave the mRNAs transcribed from these genes thereby converting the phenotype of drug-resistant cells to drug sensitivity, Increasing the EGS-to-target mRNA ratio by changing gene copy number or the number of EGSs complementary to different target sites enhances the efficiency of the conversion process, We demonstrate a general method for the efficient phenotypic conversion of drug-resistant bacterial cultures.
引用
收藏
页码:8468 / 8472
页数:5
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