Isocratic liquid chromatographic method for the analysis of azithromycin and its structurally related, substances in bulk samples

被引：18

作者：

Kamau, FN ^{[1
]}

Chepkwony, HK ^{[1
]}

Ngugi, JK ^{[1
]}

Debremaeker, D ^{[1
]}

Roets, E ^{[1
]}

Hoogmartens, J ^{[1
]}

机构：

[1] Catholic Univ Louvain, Fac Farmaceut, Lab Farmaceut Chem & Anal van Geneesmiddelen, B-3000 Louvain, Belgium

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2002年 / 40卷 / 09期

关键词：

D O I：

10.1093/chromsci/40.9.529

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An isocratic liquid chromatographic method with UV detection at 215 nm, which is suitable for the analysis of azithromycin (AZT) in bulk samples, is described. AZT is separated from its synthesis intermediates and a degradation product as well as from six unknown impurities on an XTerra RP18 column at 70°C using a mobile phase consisting of acetonitrile-pH 6.5 0.2M K2HPO4-water (35:10:55, v/v/v) at 1.0 mL/min. The XTerra stationary phase contains methyl groups that are incorporated in the bulk structure of the material. This allows for special selectivities. Robustness is evaluated by a full factorial design experiment. The method shows good selectivity, repeatability, linearity, and sensitivity.

引用

页码：529 / 533

页数：5

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