Methylated DNA labels for marking objects

被引：2

作者：

Cook, LJ ^{[1
]}

Cox, JPL ^{[1
]}

机构：

[1] Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 01期

关键词：

DNA labels; DNA methyltransferase; restriction endonuclease; restriction-modification system;

D O I：

10.1023/A:1021713625176

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We recently described a method for digitally labelling objects with DNA. Here we show that, using DNA methyltransferases to create polymorphic DNA templates, it is possible to significantly increase the number of labels that can be generated by this method. Nine double-stranded DNA templates of different length were methylated with either M. HaeIII or M. AluI methyltransferase, or both. Different mixtures of methylated and unmethylated versions of this template set were used to 'invisibly' label paper. The mixtures were eluted from the paper and the methylated status of the templates in each mixture successfully determined, and the labels read, by digestion with the complementary restriction endonuclease, followed by a polymerase chain reaction and agarose gel electrophoresis. One methylated DNA label was read after it had been left on paper for two months.

引用

页码：89 / 94

页数：6

共 7 条

[1] Bar coding objects with DNA [J].