Transcription factor distribution in Escherichia coli:: studies with FNR protein

被引:101
作者
Grainger, David C. [1 ]
Aiba, Hirofumi
Hurd, Douglas
Browning, Douglas F.
Busby, Stephen J. W.
机构
[1] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England
[2] Nagoya Univ, Sch Agr, Mol Microbiol Lab, Chikusa Ku, Nagoya, Aichi 4648601, Japan
[3] Oxford Gene Technol, Oxford OX5 1PF, England
基金
英国惠康基金;
关键词
D O I
10.1093/nar/gkl1023
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using chromatin immunoprecipitation (ChIP) and high-density microarrays, we have measured the distribution of the global transcription regulator protein, FNR, across the entire Escherichia coli chromosome in exponentially growing cells. Sixty-three binding targets, each located at the 5' end of a gene, were identified. Some targets are adjacent to poorly transcribed genes where FNR has little impact on transcription. In stationary phase, the distribution of FNR was largely unchanged. Control experiments showed that, like FNR, the distribution of the nucleoid-associated protein, IHF, is little altered when cells enter stationary phase, whilst RNA polymerase undergoes a complete redistribution.
引用
收藏
页码:269 / 278
页数:10
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