Splenic stromal cells mediate IL-7 independent adult lymphoid tissue inducer cell survival

被引：11

作者：

Hou, Tie Zheng ^{[1
]}

Mustafa, Mohammad Z. ^{[1
]}

Flavell, Sarah J. ^{[1
]}

Barrington, Fern ^{[1
]}

Jenkinson, Eric J. ^{[1
]}

Anderson, Graham ^{[1
]}

Lane, Peter J. L. ^{[1
]}

Withers, David R. ^{[1
]}

Buckley, Christopher D. ^{[1
]}

机构：

[1] Univ Birmingham, Coll Med & Dent Sci, MRC Ctr Immune Regulat,Inst Biomed Res, Rheumatol Res Grp,Sch Immun & Infect, Birmingham B15 2TT, W Midlands, England

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 2010年 / 40卷 / 02期

基金：

英国惠康基金;

关键词：

Fibroblast; Lymphoid tissue; Spleen; Stromal cells; FIBROBLASTIC RETICULAR CELLS; CD4(+)CD3(-) CELLS; T-CELL; OX40; LIGAND; NK CELLS; NODES; GENE; CD30;

D O I：

10.1002/eji.200939776

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Lymphoid tissue inducer cells (LTi) play an important role in the development of lymphoid tissue in embryos. Adult CD4(+)CD3(-) LTi-like cells present a similar phenotype and gene expression to their embryonic counterpart and have important roles in CD4(+) T-cell memory and lymphoid tissue recovery following viral infection. However, adult LTi-like cells are heterogeneous populations and the factors that regulate their survival and accumulation within secondary lymphoid organs remain unclear, in particular whether the T-zone stroma is involved. Here we report the identification and characterization of a distinct subset of podoplanin(+) murine splenic stromal cells that support adult LTi-like cell survival. We have identified and isolated CD45(-)podoplanin(+) stromal cell populations which have a similar but distinct phenotype to T-zone reticular cells in LN. CD45(-)podoplanin(+) fibroblast-like cells mediate LTi-like cell survival in vitro; surprisingly this was not dependent upon IL-7 as revealed through blocking Ab experiments and studies using LTi-like cells unable to respond to 7 chain cytokines. our findings show that adult LTi-like cells require extrinsic signals from podoplanin(+) splenic stromal cells to survive and suggest that IL-7 is not necessary to mediate their survival in the adult spleen.

引用

页码：359 / 365

页数：7

共 23 条

[11] OX40 ligand and CD30 ligand are expressed on adult but not neonatal CD4+CD3- inducer cells:: Evidence that IL-7 signals regulate CD30 ligand but not OX40 ligand expression [J].