A new antibody immobilization strategy based on electro-deposition of gold nanoparticles and Prussian Blue for label-free amperometric immunosensor

被引：33

作者：

He, Xiulan ^{[1
]}

Yuan, Ruo ^{[1
]}

Chai, Yaqin ^{[1
]}

Zhang, Ying ^{[1
]}

Shi, Yintao ^{[1
]}

机构：

[1] SW Univ, Key Lab Analyt Chem Chongqing, Coll Chem & Chem Engn, Chongqing 400715, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 01期

基金：

中国国家自然科学基金;

关键词：

amperometric immunosensor; electro-deposition; gold nanoparticle; hepatitis B surface antigen; Prussian Blue;

D O I：

10.1007/s10529-006-9211-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel and sensitive immunosensor has been developed by electro-depositing gold nanoparticles on to a Prussian Blue-modified glassy carbon electrode for determination of hepatitis B surface antigen (HBsAg). After the developed immunosensor was incubated with different concentrations of HBsAg samples at 37 degrees C for 15 min, the current response decreased with an increasing HBsAg concentration in the sample solution. The decreased percentage of the current was proportional to HBsAg concentration ranging from 2 to 300 ng HbsAg ml(-1) with a detection limit of 0.42 ng HbsAg ml(-1) (S/N = 3). Analytical results of 50 specimens using the developed immunosensor showed satisfactory agreement with those using ELISA, indicating the method to be a promising alternative for detecting HBsAg in clinical diagnosis.

引用

页码：149 / 155

页数：7

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