共 24 条
Atomic force microscopy reveals the mechanical design of a modular protein
被引:242
作者:

Li, HB
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

Oberhauser, AF
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

Fowler, SB
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

论文数: 引用数:
h-index:
机构:

Fernandez, JM
论文数: 0 引用数: 0
h-index: 0
机构:
Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA
机构:
[1] Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA
[2] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England
来源:
关键词:
D O I:
10.1073/pnas.120048697
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
Tandem modular proteins underlie the elasticity of natural adhesives, cell adhesion proteins, and muscle proteins. The fundamental unit of elastic proteins is their individually folded modules. Here, we use protein engineering to construct multimodular proteins composed of Ig modules of different mechanical strength. We examine the mechanical properties of the resulting tandem modular proteins by using single protein atomic force microscopy. We show that by combining modules of known mechanical strength, we can generate proteins with novel elastic properties. Our experiments reveal the simple mechanical design of modular proteins and open the way for the engineering of elastic proteins with defined mechanical properties, which can be used in tissue and fiber engineering.
引用
收藏
页码:6527 / 6531
页数:5
相关论文
共 24 条
[11]
Unfolding of titin immunoglobulin domains by steered molecular dynamics simulation
[J].
Lu, H
;
Isralewitz, B
;
Krammer, A
;
Vogel, V
;
Schulten, K
.
BIOPHYSICAL JOURNAL,
1998, 75 (02)
:662-671

Lu, H
论文数: 0 引用数: 0
h-index: 0
机构: Univ Illinois, Beckman Inst Adv Sci & Technol, Urbana, IL 61801 USA

Isralewitz, B
论文数: 0 引用数: 0
h-index: 0
机构: Univ Illinois, Beckman Inst Adv Sci & Technol, Urbana, IL 61801 USA

Krammer, A
论文数: 0 引用数: 0
h-index: 0
机构: Univ Illinois, Beckman Inst Adv Sci & Technol, Urbana, IL 61801 USA

论文数: 引用数:
h-index:
机构:

Schulten, K
论文数: 0 引用数: 0
h-index: 0
机构: Univ Illinois, Beckman Inst Adv Sci & Technol, Urbana, IL 61801 USA
[12]
Mechanical unfolding intermediates in titin modules
[J].
Marszalek, PE
;
Lu, H
;
Li, HB
;
Carrion-Vazquez, M
;
Oberhauser, AF
;
Schulten, K
;
Fernandez, JM
.
NATURE,
1999, 402 (6757)
:100-103

Marszalek, PE
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

Lu, H
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

Li, HB
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

Carrion-Vazquez, M
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

Oberhauser, AF
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

Schulten, K
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

Fernandez, JM
论文数: 0 引用数: 0
h-index: 0
机构:
Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA
[13]
New measurements of DNA twist elasticity
[J].
Nelson, P
.
BIOPHYSICAL JOURNAL,
1998, 74 (05)
:2501-2503

Nelson, P
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Penn, Dept Phys & Astron, Philadelphia, PA 19104 USA Univ Penn, Dept Phys & Astron, Philadelphia, PA 19104 USA
[14]
The molecular elasticity of the extracellular matrix protein tenascin
[J].
Oberhauser, AF
;
Marszalek, PE
;
Erickson, HP
;
Fernandez, JM
.
NATURE,
1998, 393 (6681)
:181-185

Oberhauser, AF
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

Marszalek, PE
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

Erickson, HP
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

Fernandez, JM
论文数: 0 引用数: 0
h-index: 0
机构:
Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA
[15]
Dynamics and elasticity of the fibronectin matrix in living cell culture visualized by fibronectin-green fluorescent protein
[J].
Ohashi, T
;
Kiehart, DP
;
Erickson, HP
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
1999, 96 (05)
:2153-2158

Ohashi, T
论文数: 0 引用数: 0
h-index: 0
机构:
Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA

Kiehart, DP
论文数: 0 引用数: 0
h-index: 0
机构:
Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA

Erickson, HP
论文数: 0 引用数: 0
h-index: 0
机构:
Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA
[16]
The elastic I-band region of titin is assembled in a ''modular'' fashion by weakly interacting Ig-like domains
[J].
Politou, AS
;
Gautel, M
;
Improta, S
;
Vangelista, L
;
Pastore, A
.
JOURNAL OF MOLECULAR BIOLOGY,
1996, 255 (04)
:604-616

Politou, AS
论文数: 0 引用数: 0
h-index: 0
机构: Europ. Molecular Biology Laboratory, Heidelberg, 69117

Gautel, M
论文数: 0 引用数: 0
h-index: 0
机构: Europ. Molecular Biology Laboratory, Heidelberg, 69117

Improta, S
论文数: 0 引用数: 0
h-index: 0
机构: Europ. Molecular Biology Laboratory, Heidelberg, 69117

Vangelista, L
论文数: 0 引用数: 0
h-index: 0
机构: Europ. Molecular Biology Laboratory, Heidelberg, 69117

Pastore, A
论文数: 0 引用数: 0
h-index: 0
机构: Europ. Molecular Biology Laboratory, Heidelberg, 69117
[17]
The folding and stability of titin immunoglobulin-like modules, with implications for the mechanism of elasticity
[J].
Politou, AS
;
Thomas, DJ
;
Pastore, A
.
BIOPHYSICAL JOURNAL,
1995, 69 (06)
:2601-2610

Politou, AS
论文数: 0 引用数: 0
h-index: 0
机构: European Molecular Biology Laboratory, Heidelberg

Thomas, DJ
论文数: 0 引用数: 0
h-index: 0
机构: European Molecular Biology Laboratory, Heidelberg

Pastore, A
论文数: 0 引用数: 0
h-index: 0
机构: European Molecular Biology Laboratory, Heidelberg
[18]
Reversible unfolding of individual titin immunoglobulin domains by AFM
[J].
Rief, M
;
Gautel, M
;
Oesterhelt, F
;
Fernandez, JM
;
Gaub, HE
.
SCIENCE,
1997, 276 (5315)
:1109-1112

Rief, M
论文数: 0 引用数: 0
h-index: 0
机构: LEHRSTUHL ANGEW PHYS,D-80799 MUNICH,GERMANY

Gautel, M
论文数: 0 引用数: 0
h-index: 0
机构: LEHRSTUHL ANGEW PHYS,D-80799 MUNICH,GERMANY

Oesterhelt, F
论文数: 0 引用数: 0
h-index: 0
机构: LEHRSTUHL ANGEW PHYS,D-80799 MUNICH,GERMANY

Fernandez, JM
论文数: 0 引用数: 0
h-index: 0
机构: LEHRSTUHL ANGEW PHYS,D-80799 MUNICH,GERMANY

Gaub, HE
论文数: 0 引用数: 0
h-index: 0
机构: LEHRSTUHL ANGEW PHYS,D-80799 MUNICH,GERMANY
[19]
Elastically coupled two-level systems as a model for biopolymer extensibility
[J].
Rief, M
;
Fernandez, JM
;
Gaub, HE
.
PHYSICAL REVIEW LETTERS,
1998, 81 (21)
:4764-4767

Rief, M
论文数: 0 引用数: 0
h-index: 0
机构: Lehrstuhl Angew Phys, D-80799 Munich, Germany

Fernandez, JM
论文数: 0 引用数: 0
h-index: 0
机构: Lehrstuhl Angew Phys, D-80799 Munich, Germany

Gaub, HE
论文数: 0 引用数: 0
h-index: 0
机构: Lehrstuhl Angew Phys, D-80799 Munich, Germany
[20]
Molecular mechanistic origin of the toughness of natural adhesives, fibres and composites
[J].
Smith, BL
;
Schäffer, TE
;
Viani, M
;
Thompson, JB
;
Frederick, NA
;
Kindt, J
;
Belcher, A
;
Stucky, GD
;
Morse, DE
;
Hansma, PK
.
NATURE,
1999, 399 (6738)
:761-763

Smith, BL
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

Schäffer, TE
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

Viani, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

Thompson, JB
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

Frederick, NA
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

Kindt, J
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

Belcher, A
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

Stucky, GD
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

Morse, DE
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

Hansma, PK
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA