A molecular imprinted polymer with recognition properties towards the carcinogenic mycotoxin ochratoxin A

被引:55
作者
Baggiani, C [1 ]
Giraudi, G [1 ]
Vanni, A [1 ]
机构
[1] Univ Turin, Dipartimento Chim Analit, I-10125 Turin, Italy
关键词
molecular imprinting; ochratoxin A; mycotoxin; liquid chromatography; molecular recognition;
D O I
10.1023/A:1021506223136
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A molecularly imprinted polymer which recognises the mycotoxin ochratoxin A was prepared using the mimic N-(4-chloro-1-hydroxy-2-naphthoylamido)-(L)-phenylalanineas a template. The polymer was obtained by dissolving the template, methacrylic acid and ethylendimethacrylate in chloroform and polymerising the mixture by thermal treatment at 60degreesC. The monolith obtained was crushed, sieved to 30-90 mum and extensively washed till the template could no longer be found in the washing solution. The binding properties towards the template, ochratoxin A and several related molecules were measured by eluting with acetonitrile and chloroform a HPLC column packed with the imprinted polymer. The experimental results show that the polymer recognises not only the template well, but also the ochratoxin A. The specific molecular recognition effect is due to hydrogen bond interactions but in order to assure the full recognition effect adjunctive steric factors are necessary. The magnitude of these interactions can be controlled by the use of limited amounts of acetic acid in the mobile phase. From the measurement of the relative selectivity it was found that only the simultaneous presence of the carboxyl, the phenolic hydroxyl and certain peculiar substructures such as the chlorine atom assures the whole recognition of the template.
引用
收藏
页码:389 / 394
页数:6
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