Detection of pathological molecular alterations in scrapie-infected hamster brain by Fourier transform infrared (FT-IR) spectroscopy

被引：108

作者：

Kneipp, J ^{[1
]}

Lasch, P ^{[1
]}

Baldauf, E ^{[1
]}

Beekes, M ^{[1
]}

Naumann, D ^{[1
]}

机构：

[1] Robert Koch Inst, D-13353 Berlin, Germany

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | 2000年 / 1501卷 / 2-3期

关键词：

scrapie strain 263K; transmissible spongiform encephalopathy; microspectroscopy; spectral mapping; cerebellum; medulla oblongata; pons;

D O I：

10.1016/S0925-4439(00)00021-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this report a new approach for the identification of pathological changes in scrapie-infected Syrian hamster brains using Fourier transform infrared microspectroscopy is discussed. Using computer-based pattern recognition techniques and imaging, infrared maps with high structural contrast were obtained. This strategy permitted comparison of spectroscopic data from identical anatomical structures in scrapie-infected and control brains. Consistent alterations in membrane state-of-order, protein composition, carbohydrate and nucleic acid constituents were detected in scrapie-infected tissues. Cluster analysis performed on spectra of homogenized medulla oblongata and pens samples also reliably separated uninfected from infected specimens. This method provides a useful tool not only for the exploration of the disease process but also for the development of rapid diagnostic and screening techniques of transmissible spongiform encephalopathies. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：189 / 199

页数：11

共 25 条

[1] WESTERN-BLOT MAPPING OF DISEASE-SPECIFIC AMYLOID IN VARIOUS ANIMAL SPECIES AND HUMANS WITH TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES USING A HIGH-YIELD PURIFICATION METHOD [J].