Quantitative detection and differentiation of free-living amoeba species using SYBR green-based real-time PCR melting curve analysis

被引：17

作者：

Behets, Jonas

Declerck, Priscilla

Delaedt, Yasmine

Verelst, Lieve

Ollevier, Frans

机构：

[1] Catholic Univ Louvain VIB, Aquat Ecol Lab, B-3000 Louvain, Belgium

[2] LABORELEC, B-1630 Linkebeek, Belgium

来源：

CURRENT MICROBIOLOGY | 2006年 / 53卷 / 06期

关键词：

D O I：

10.1007/s00284-006-0241-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Real-time polymerase chain reaction melting curve analysis (MCA) allows differentiation of several free-living amoebae species. Distinctive characteristics were found for Naegleria fowleri, N. lovaniensis, N. australiensis, N. gruberi, Hartmanella vermiformis, and Willaertia magna. Species specificity of the amplicons was confirmed using agarose gel electrophoresis and sequence-based approaches. Amplification efficiency ranged from 91% to 98%, indicating the quantitative potential of the assay. This MCA approach can be used for quantitative detection of free-living amoebae after cultivation but also as a culture-independent detection method.

引用

页码：506 / 509

页数：4

共 23 条

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