Capillary electrophoretic resolution of the 2′-, 3′- and 5′-isomers of nucleotide monophosphates via the zinc complexes

The affinity of nucleotides for zinc ion enables resolution of the 2'-, 3'- and 5'-isomers of nucleotide monophosphates. The concentration of zinc ion modifies the electrophoretic mobilities of nucleoside monophosphates differently depending on the nature of the base and the position (2', 3' and 5') of the phosphate group on the sugar moiety. Fast co-electroosmotic separation (5 min) of twelve nucleotide was performed with an acidic citrate-phosphate buffer to which zinc ion has been added.