Functional transcranial brain imaging by optical-resolution photoacoustic microscopy

被引:139
作者
Hu, Song [1 ]
Maslov, Konstantin [1 ]
Tsytsarev, Vassiliy [1 ]
Wang, Lihong V. [1 ]
机构
[1] Washington Univ, Dept Biomed Engn, Opt Imaging Lab, St Louis, MO 63130 USA
基金
美国国家卫生研究院;
关键词
optical-resolution photoacoustic microscopy; transcranial brain imaging; label-free; capillary; hemoglobin oxygen saturation; vascular branching; cortical plasticity; neurovascular coupling; TISSUE;
D O I
10.1117/1.3194136
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Optical-resolution photoacoustic microscopy (OR-PAM) is applied to functional brain imaging in living mice. A near-diffraction-limited bright-field optical illumination is employed to achieve micrometer lateral resolution, and a dual-wavelength measurement is utilized to extract the blood oxygenation information. The variation in hemoglobin oxygen saturation (sO(2)) along vascular branching has been imaged in a precapillary arteriolar tree and a postcapillary venular tree, respectively. To the best of our knowledge, this is the first report on in vivo volumetric imaging of brain microvascular morphology and oxygenation down to single capillaries through intact mouse skulls. It is anticipated that: (i) chronic imaging enabled by this minimally invasive procedure will advance the study of cortical plasticity and neurological diseases; (ii) revealing the neuroactivity-dependent changes in hemoglobin concentration and oxygenation will facilitate the understanding of neurovascular coupling at the capillary level; and (iii) combining functional OR-PAM and high-resolution blood flowmetry will have the potential to explore cellular pathways of brain energy metabolism. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3194136]
引用
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页数:3
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